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. 2012 Mar;12(1):61-5.
doi: 10.1007/s10238-011-0138-5. Epub 2011 May 13.

Gene expression of O-GlcNAc cycling enzymes in human breast cancers

Anna Krześlak  1 , Ewa Forma, Magdalena Bernaciak, Hanna Romanowicz, Magdalena Bryś
Affiliations

Gene expression of O-GlcNAc cycling enzymes in human breast cancers

Anna Krześlak et al. Clin Exp Med. 2012 Mar.

Abstract

O-GlcNAcylation is an abundant, dynamic, and inducible posttranslational modification in which single β-N-acetylglucosamine residues are attached by O-glycosidic linkage to serine or treonine residues. It is suggested that abnormally regulated O-GlcNAcylation may contribute to the pathology of cancer. Cycling of O-GlcNAc residues on intracellular proteins is controlled by two enzymes, O-GlcNAc transferease (OGT), which catalyses the addition of O-GlcNAc residues and nucleocytoplasmic β-N-acetylglucosaminidase (O-GlcNAcase; encoded by MGEA5 gene), an enzyme involved in the removal of O-GlcNAc. In this study, relationship between the mRNA expressions of genes coding O-GlcNAc cycling enzymes in breast ductal carcinomas and clinicopathological parameters were analyzed. The results showed that poorly differentiated tumors (grade II and III) had significantly higher OGT expression than grade I tumors. Contrary, MGEA5 transcript levels were significantly lower in grade II and III in comparison with grade I tumors. The Spearman rank correlation showed the expressions of OGT and MGEA5 in breast cancer was negatively correlated (r = -0.430, P = 0.0002). Lymph node metastasis status was significantly associated with decreased MGEA5 mRNA expression. This result suggests that elevation in O-GlcNAc modification of proteins may be implicated in breast tumor progression and metastasis.

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Figures

Fig. 1
Fig. 1
OGT and MGEA5 mean mRNA expression level in ductal breast cancers; a comparison between subgroups with different tumor grade, lymph node metastasis status, and estrogen/progesterone status. Error bars represents standard error; *P < 0.05, **P < 0.01, ***P < 0.001
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