doi: 10.1016/0014-5793(90)80659-7.
Sphingomyelin is synthesized in the cis Golgi
Affiliations
- PMID: 2155131
- DOI: 10.1016/0014-5793(90)80659-7
Item in Clipboard
Sphingomyelin is synthesized in the cis Golgi
FEBS Lett.
.
Free article
Abstract
We have employed in vitro a truncated ceramide analogue with 8 carbon atoms in the sphingosine and the fatty acyl residue, each, to investigate the activity of various membrane fractions to synthesize truncated sphingomyelin. This shortened ceramide readily diffuses through membranes and therefore can easily find access to the lumina of intact organelles. Sphingomyelin synthase activity resides in the Golgi apparatus, and after sucrose density gradient centrifugation of Golgi-enriched fractions sphingomyelin synthesis follows a cis Golgi marker enzyme.
Similar articles
-
The subcellular sites of sphingomyelin synthesis in BHK cells.Biochim Biophys Acta. 1997 Oct 30;1359(1):1-12. doi: 10.1016/s0167-4889(97)00088-8. Biochim Biophys Acta. 1997. PMID: 9398080
-
Preparation of Golgi subfractions with free-solution isotachophoresis: analysis of sphingomyelin synthesis in Golgi subfractions from rat liver.Electrophoresis. 1998 Jun;19(7):1185-94. doi: 10.1002/elps.1150190720. Electrophoresis. 1998. PMID: 9662182
-
Sphingomyelin synthesis in rat liver occurs predominantly at the cis and medial cisternae of the Golgi apparatus.J Biol Chem. 1990 May 25;265(15):8650-7. J Biol Chem. 1990. PMID: 2187869
-
Inhibitors of the sphingomyelin cycle: Sphingomyelin synthases and sphingomyelinases.Chem Phys Lipids. 2016 May;197:45-59. doi: 10.1016/j.chemphyslip.2015.07.008. Epub 2015 Jul 19. Chem Phys Lipids. 2016. PMID: 26200918 Review.
-
Biosynthesis of sphingomyelin and its delivery to the surface of baby hamster kidney (BHK) cells.Biochem Soc Trans. 1993 May;21(2):240-4. doi: 10.1042/bst0210240. Biochem Soc Trans. 1993. PMID: 8359473 Review. No abstract available.
Cited by
-
Deficiency of sphingomyelin synthase 1 but not sphingomyelin synthase 2 reduces bone formation due to impaired osteoblast differentiation.Mol Med. 2019 Dec 17;25(1):56. doi: 10.1186/s10020-019-0123-0. Mol Med. 2019. PMID: 31847800 Free PMC article.
-
The domain responsible for sphingomyelin synthase (SMS) activity.Biochim Biophys Acta. 2008 Oct;1781(10):610-7. doi: 10.1016/j.bbalip.2008.07.002. Epub 2008 Jul 23. Biochim Biophys Acta. 2008. PMID: 18694848 Free PMC article.
-
Identification of a family of animal sphingomyelin synthases.EMBO J. 2004 Jan 14;23(1):33-44. doi: 10.1038/sj.emboj.7600034. Epub 2003 Dec 18. EMBO J. 2004. PMID: 14685263 Free PMC article.
-
Ceramide Transfer Protein (CERT): An Overlooked Molecular Player in Cancer.Int J Mol Sci. 2021 Dec 7;22(24):13184. doi: 10.3390/ijms222413184. Int J Mol Sci. 2021. PMID: 34947980 Free PMC article. Review.
-
Ceramide transport from endoplasmic reticulum to Golgi apparatus is not vesicle-mediated.Biochem J. 1998 Aug 1;333 ( Pt 3)(Pt 3):779-86. doi: 10.1042/bj3330779. Biochem J. 1998. PMID: 9677340 Free PMC article.
