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. 1990 Feb;174(2):605-7.
doi: 10.1016/0042-6822(90)90114-7.

Mouse hepatitis virus ORF 2a is expressed in the cytosol of infected mouse fibroblasts

P W Zoltick  1 , J L Leibowitz, E L Oleszak, S R Weiss
Affiliations

Mouse hepatitis virus ORF 2a is expressed in the cytosol of infected mouse fibroblasts

P W Zoltick et al. Virology. 1990 Feb.

Abstract

A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17Cl-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.

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