Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Oct;87(20):7926-9.
doi: 10.1073/pnas.87.20.7926.

Accumulation of p21ras.GTP in response to stimulation with epidermal growth factor and oncogene products with tyrosine kinase activity

T Satoh  1 , M Endo, M Nakafuku, T Akiyama, T Yamamoto, Y Kaziro
Affiliations

Accumulation of p21ras.GTP in response to stimulation with epidermal growth factor and oncogene products with tyrosine kinase activity

T Satoh et al. Proc Natl Acad Sci U S A. 1990 Oct.

Abstract

The ras gene product (p21) is a GTP-binding protein and has been thought to transduce signals regulating proliferation or differentiation of cells. Like other GTP-binding proteins, p21.GTP is an active conformation, which can transduce the signals downstream, whereas p21.GDP is an inactive one. Recently, we have shown that p21.GTP levels increased in cells treated with fetal bovine serum or platelet-derived growth factor to initiate DNA synthesis. In this paper, we report that epidermal growth factor can also increase the amounts of p21.GTP in the cells. Effects of epidermal growth factor and platelet-derived growth factor are not additive. In contrast, mutant [Val12]p21, which has transforming activity, responded neither to platelet-derived growth factor nor to epidermal growth factor. We also found that the ratio of p21.GTP to p21.GDP increased 3- to 4-fold in transformants carrying activated erbB-2/neu or v-src oncogenes. These results strongly suggest an important role of p21 in transduction of signals for both normal proliferation and malignant transformation through growth factor receptors with tyrosine kinase activity or related oncogene products.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochim Biophys Acta. 1978 Sep 21;505(1):95-127 - PubMed
    1. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5993-7 - PubMed
    1. Nature. 1985 Jan 17-23;313(5999):241-3 - PubMed
    1. Nature. 1986 Apr 10-16;320(6062):540-3 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jan;84(2):546-50 - PubMed

MeSH terms

LinkOut - more resources