Sorting pathways of mitochondrial inner membrane proteins
- PMID: 2145157
- DOI: 10.1111/j.1432-1033.1990.tb19260.x
Sorting pathways of mitochondrial inner membrane proteins
Abstract
Two distinct pathways of sorting and assembly of nuclear-encoded mitochondrial inner membrane proteins are described. In the first pathway, precursor proteins that carry amino-terminal targeting signals are initially translocated via contact sites between both mitochondrial membranes into the mitochondrial matrix. They become proteolytically processed, interact with the 60-kDa heat-shock protein hsp60 in the matrix and are retranslocated to the inner membrane. The sorting of subunit 9 of Neurospora crassa F0-ATPase has been studied as an example. F0 subunit 9 belongs to that class of nuclear-encoded mitochondrial proteins which are evolutionarily derived from a prokaryotic ancestor according to the endosymbiont hypothesis. We suggest that after import into mitochondria, these proteins follow the ancestral sorting and assembly pathways established in prokaryotes (conservative sorting). On the other hand, ADP/ATP carrier was found not to require interaction with hsp60 for import and assembly. This agrees with previous findings that the ADP/ATP carrier possesses non-amino-terminal targeting signals and uses a different import receptor to other mitochondrial precursor proteins. It is proposed that the ADP/ATP carrier represents a class of mitochondrial inner membrane proteins which do not have a prokaryotic equivalent and thus appear to follow a non-conservative sorting pathway.
Similar articles
-
Conservative sorting of F0-ATPase subunit 9: export from matrix requires delta pH across inner membrane and matrix ATP.EMBO J. 1995 Jul 17;14(14):3445-51. doi: 10.1002/j.1460-2075.1995.tb07350.x. EMBO J. 1995. PMID: 7628445 Free PMC article.
-
Early events in the transport of proteins into mitochondria. Import competition by a mitochondrial presequence.J Biol Chem. 1991 Nov 15;266(32):21700-8. J Biol Chem. 1991. PMID: 1834661
-
The carboxyl-terminal two-thirds of the ADP/ATP carrier polypeptide contains sufficient information to direct translocation into mitochondria.J Biol Chem. 1987 Nov 5;262(31):14851-4. J Biol Chem. 1987. PMID: 2822702
-
Mitochondrial protein import.Biochim Biophys Acta. 1989 Jan 18;988(1):1-45. doi: 10.1016/0304-4157(89)90002-6. Biochim Biophys Acta. 1989. PMID: 2642391 Review.
-
Mitochondrial protein import: mechanisms, components and energetics.Biochim Biophys Acta. 1994 Aug 30;1187(2):270-4. doi: 10.1016/0005-2728(94)90125-2. Biochim Biophys Acta. 1994. PMID: 8075121 Review.
Cited by
-
Intramitochondrial sorting of the precursor to yeast cytochrome c oxidase subunit Va.J Cell Biol. 1993 Jun;121(5):1021-9. doi: 10.1083/jcb.121.5.1021. J Cell Biol. 1993. PMID: 8388875 Free PMC article.
-
Identification of the gene encoding the mitochondrial elongation factor G in mammals.Nucleic Acids Res. 1993 Jun 11;21(11):2641-7. doi: 10.1093/nar/21.11.2641. Nucleic Acids Res. 1993. PMID: 8332461 Free PMC article.
-
Mitochondrial protein import: specific recognition and membrane translocation of preproteins.J Membr Biol. 1993 Sep;135(3):191-207. doi: 10.1007/BF00211091. J Membr Biol. 1993. PMID: 8271259 Review. No abstract available.
-
Import of cytochrome c heme lyase into mitochondria: a novel pathway into the intermembrane space.EMBO J. 1992 Feb;11(2):449-56. doi: 10.1002/j.1460-2075.1992.tb05074.x. EMBO J. 1992. PMID: 1371459 Free PMC article.
-
The sorting signal of cytochrome b2 promotes early divergence from the general mitochondrial import pathway and restricts the unfoldase activity of matrix Hsp70.EMBO J. 1995 Dec 1;14(23):6043-57. doi: 10.1002/j.1460-2075.1995.tb00293.x. EMBO J. 1995. PMID: 8846797 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
