doi: 10.1016/0014-5793(90)80243-c.
Microtubule translocation caused by three subspecies of inner-arm dynein from Chlamydomonas flagella
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- PMID: 2141576
- DOI: 10.1016/0014-5793(90)80243-c
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Microtubule translocation caused by three subspecies of inner-arm dynein from Chlamydomonas flagella
FEBS Lett.
.
Free article
Abstract
To help understand the function of inner-arm dynein in flagellar motility, dynein samples from an outer arm-missing mutant of Chlamydomonas (oda1) were examined for the ability to translocate microtubules in vitro. High-salt extract of axonemes containing inner-arm dynein was separated by ion-exchange chromatography into 7 peak fractions with ATPase activities. Of these, three fractions containing different sets of dynein heavy chains translocated microtubules. The maximal velocities were all between 3 and 5 microns/s, which were comparable to the microtubule sliding rate in disintegrating oda axonemes.
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