Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Mar;87(5):2008-12.
doi: 10.1073/pnas.87.5.2008.

An adenovirus E4 gene product trans-activates E2 transcription and stimulates stable E2F binding through a direct association with E2F

S D Neill  1 , C Hemstrom, A Virtanen, J R Nevins
Affiliations

An adenovirus E4 gene product trans-activates E2 transcription and stimulates stable E2F binding through a direct association with E2F

S D Neill et al. Proc Natl Acad Sci U S A. 1990 Mar.

Abstract

The adenovirus E4 gene encodes a trans-activating function that can stimulate the E2 promoter. E2 promoter sequences required for E4 trans-activation are identical to those required for E1A trans-activation, and these principally are the E2 promoter binding factor (E2F) binding sites. Furthermore, full activation of E2F DNA binding activity requires both E1A and E4 action. Analysis of a series of mutant E4 viruses identifies open reading frame (orf) 6/7 of the E4 transcription unit as that required for activation of E2F binding activity. In addition, the assay of various E4 cDNAs demonstrates that the E4 orf 6/7 also is responsible for the trans-activation of E2 transcription. Translation of the E4 orf 6/7 mRNA, but not a control mRNA, in a reticulocyte extract generates an activity that can stimulate cooperative binding of E2F in vitro, consistent with recent in vivo assays that demonstrate a role for the E4 gene in E2F stable complex formation. This stimulation is due to a direct interaction of the E4 protein with E2F since an antibody that recognizes the E4 orf 6/7 polypeptide detects this E4 protein in the E2F-DNA complex. We conclude that the E4 orf 6/7 product interacts with the E2F factor altering binding to allow formation of a stable complex that results in a stimulation of transcription.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Genes Dev. 1988 May;2(5):578-87 - PubMed
    1. Genes Dev. 1989 Nov;3(11):1699-710 - PubMed
    1. Mol Cell Biol. 1982 Sep;2(9):1044-51 - PubMed
    1. J Mol Biol. 1982 Dec 15;162(3):565-83 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5383-6 - PubMed

Publication types

LinkOut - more resources