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. 2011 Mar;32(3):385-92.
doi: 10.1038/aps.2010.214.

Establishment and characterization of primary lung cancer cell lines from Chinese population

Chao Zheng  1 , Yi-hua Sun, Xiao-lei Ye, Hai-quan Chen, Hong-bin Ji
Affiliations

Establishment and characterization of primary lung cancer cell lines from Chinese population

Chao Zheng et al. Acta Pharmacol Sin. 2011 Mar.

Abstract

Aim: To establish and characterize primary lung cancer cell lines from Chinese population.

Methods: Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice.

Results: Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations.

Conclusion: These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response.

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Figures

Figure 1
Figure 1
Typical pathological images of the tumor tissues used to establish primary Chinese lung cancer cell lines. Typical pathological images of the original tumors of primary Chinese lung cancer cell lines are shown, including SH-416 (SCC), SH-437 (LC), SH-224 (Ad), SH-543 (Ad), SH-450 (AS), SH-405 (PDE), SH-498 (SCLC).
Figure 2
Figure 2
Representative morphology of primary Chinese lung cancer cell lines under light microscope. Typical morphology of cell lines is shown, including SH-224 (Ad), SH-289 (Ad), SH-416 (SCC), SH-437 (LC), SH-450 (AS), SH-543 (Ad), SH-405 (PDE), and SH-498 (SCLC).
Figure 3
Figure 3
Ploidy and cytogenetic analyses of primary Chinese lung cancer cell lines. (A) Ploidy distribution of six Chinese NSCLC cell lines. (B) The summary of chromosome counting in the six Chinese NSCLC cell lines. (C) The karyotype of SH-543 is shown as one example.
Figure 4
Figure 4
Status of EGFR mutation and gene amplification in primary Chinese lung cancer cell lines. (A) DNA sequencing results showed EGFR mutations in the indicated cell lines. (B) FISH analyses showed either EGFR high polysomy or gene amplification in the indicated cell lines. Green: chromosome 7 centromere; Red: EGFR.
Figure 5
Figure 5
Cell growth of primary Chinese lung cancer cell lines in two dimension and soft agar. (A) The cell growth kinetics of the five adherent NSCLC cell lines are indicated. (B) The anchorage-independent cell growth of the five NSCLC cell lines. CRL-5803 serves as the control.
Figure 6
Figure 6
Tumor formation ability of primary Chinese NSCLC cell lines in nude mice. (A) The summary of tumorigenicity in four Chinese NSCLC cell lines in nude mice. (B) Representative photos are shown for the xenografts; (C) H&E staining of the tumors from xenografts.
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