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. 2011 Mar;25(3):763-8.
doi: 10.3892/or.2011.1146. Epub 2011 Jan 14.

mTOR inhibitors show promising in vitro activity in bladder cancer and head and neck squamous cell carcinoma

Fiona Schedel  1 , Ralph Pries, Beate Thode, Brigitte Wollmann, Sandra Wulff, Dieter Jocham, Barbara Wollenberg, Ingo Kausch
Affiliations

mTOR inhibitors show promising in vitro activity in bladder cancer and head and neck squamous cell carcinoma

Fiona Schedel et al. Oncol Rep. 2011 Mar.

Abstract

Bladder cancer and head and neck squamous cell carcinoma (HNSCC) are frequent but lack efficient therapies especially in advanced disease. Almost no studies on mTOR function and inhibition in these tumor entities have been reported. We examined the gene and protein expression levels of mTOR and its activated form (pmTOR) in three human bladder carcinoma cell lines (RT-4, T24, EJ28) and three HNSCC cell lines (PCI-1, PCI-13, BHY). Furthermore, the consequences of mTOR inhibition by mTOR-specific siRNAs and the mTOR inhibitor temsirolimus were analysed in vitro using immunohistochemical Ki-67 staining, mTOR and pmTOR western blot analysis, MTT assay, as well as cell cycle analysis with flow cytometry. Especially pmTOR protein expression levels showed marked differences between cell lines. siRNA transfection was associated with dose-dependent target protein reduction but not proliferation inhibition or apoptosis. On the contrary, temsirolimus significantly reduced cell viability and induced apoptosis and cell cycle arrest. According to these data, bladder cancer and HNSCC are promising tumor entities for mTOR inhibition with temsirolimus.

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