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. 2010 Feb;1(2):198-204.
doi: 10.1007/s13238-010-0028-8. Epub 2010 Feb 6.

New nsp8 isoform suggests mechanism for tuning viral RNA synthesis

Shuang Li  1 , Qi Zhao, Yinjie Zhang, Yang Zhang, Mark Bartlam, Xuemei Li, Zihe Rao
Affiliations

New nsp8 isoform suggests mechanism for tuning viral RNA synthesis

Shuang Li et al. Protein Cell. 2010 Feb.

Abstract

During severe acute respiratory syndrome coronavirus (SARS-CoV) infection, the activity of the replication/transcription complexes (RTC) quickly peaks at 6 hours post infection (h.p.i) and then diminishes significantly in the late post-infection stages. This "down-up-down" regulation of RNA synthesis distinguishes different viral stages: primary translation, genome replication, and finally viron assembly. Regarding the nsp8 as the primase in RNA synthesis, we confirmed that the proteolysis product of the primase (nsp8) contains the globular domain (nsp8C), and indentified the resectioning site that is notably conserved in all the three groups of coronavirus. We subsequently crystallized the complex of SARS-CoV nsp8C and nsp7, and the 3-D structure of this domain revealed its capability to interfuse into the hexadecamer super-complex. This specific proteolysis may indicate one possible mechanism by which coronaviruses to switch from viral infection to genome replication and viral assembly stages.

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References

    1. Collaborative Computational Project, Number 4. The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Crystallogr. 1994;50:760–763. doi: 10.1107/S0907444994003112. - DOI - PubMed
    1. Brandt C.R. The role of viral and host genes in corneal infection with herpes simplex virus type 1. Exp Eye Res. 2005;80:607–621. doi: 10.1016/j.exer.2004.09.007. - DOI - PubMed
    1. Brockway S.M., Clay C.T., Lu X.T., Denison M.R. Characterization of the expression, intracellular localization, and replication complex association of the putative mouse hepatitis virus RNA-dependent RNA polymerase. J Virol. 2003;77:10515–10527. doi: 10.1128/JVI.77.19.10515-10527.2003. - DOI - PMC - PubMed
    1. Brunger A.T., Adams P.D., Clore G.M., DeLano W.L., Gros P., Grosse-Kunstleve R.W., Jiang J.S., Kuszewski J., Nilges M., Pannu N.S., et al. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr D. 1998;54:905–921. doi: 10.1107/S0907444998003254. - DOI - PubMed
    1. Deming D.J., Graham R.L., Denison M.R., Baric R.S. Processing of open reading frame 1a replicase proteins nsp7 to nsp10 in murine hepatitis virus strain A59 replication. J Virol. 2007;81:10280–10291. doi: 10.1128/JVI.00017-07. - DOI - PMC - PubMed

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