Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells
- PMID: 2116990
- DOI: 10.1101/gad.4.6.993
Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells
Abstract
A powerful enhancer has been mapped to an 18-bp DNA segment located 11 kb 5' to the human epsilon-globin gene within the dominant control or locus-activating region. This enhancer is inducible in K562 human erythroleukemia cells, increasing linked gamma-globin promoter/luciferase gene expression to 170-fold over an enhancerless construct. The enhancer consists of tandem AP-1-binding sites, phased 10 bp apart, which are both required for full activity. DNA-protein binding assays with nuclear extracts from induced cells demonstrate a high molecular weight complex on the enhancer. The formation of this complex also requires both AP-1 sites and correlates with maximal enhancer activity. Induction of the enhancer may have a role in the increase in globin gene transcription that characterizes erythroid maturation. Enhancer activity appears to be mediated by the binding of a complex of proteins from the jun and fos families to tandem AP-1 consensus sequences.
Similar articles
-
Inducibility of the HS II enhancer depends on binding of an erythroid specific nuclear protein.Nucleic Acids Res. 1990 Oct 25;18(20):6011-7. doi: 10.1093/nar/18.20.6011. Nucleic Acids Res. 1990. PMID: 2235483 Free PMC article.
-
A 46 base pair enhancer sequence within the locus activating region is required for induced expression of the gamma-globin gene during erythroid differentiation.Nucleic Acids Res. 1990 May 11;18(9):2721-31. doi: 10.1093/nar/18.9.2721. Nucleic Acids Res. 1990. PMID: 2339058 Free PMC article.
-
Gamma-globin gene promoter elements required for interaction with globin enhancers.Blood. 1998 Jan 1;91(1):309-18. Blood. 1998. PMID: 9414299
-
Structure and function of the enhancer 3' to the human A gamma globin gene.Nucleic Acids Res. 1990 Dec 25;18(24):7407-15. doi: 10.1093/nar/18.24.7407. Nucleic Acids Res. 1990. PMID: 2259631 Free PMC article.
-
Enhancer function in viral and cellular gene regulation.Biochim Biophys Acta. 1989 Dec 17;989(2):97-110. doi: 10.1016/0304-419x(89)90037-1. Biochim Biophys Acta. 1989. PMID: 2688749 Review. No abstract available.
Cited by
-
Structure and function of the murine beta-globin locus control region 5' HS-3.Nucleic Acids Res. 1992 Nov 11;20(21):5771-8. doi: 10.1093/nar/20.21.5771. Nucleic Acids Res. 1992. PMID: 1454538 Free PMC article.
-
Multiple regions of p45 NF-E2 are required for beta-globin gene expression in erythroid cells.Nucleic Acids Res. 1997 Jun 15;25(12):2509-15. doi: 10.1093/nar/25.12.2509. Nucleic Acids Res. 1997. PMID: 9171106 Free PMC article.
-
Mutagenesis of retroviral vectors transducing human beta-globin gene and beta-globin locus control region derivatives results in stable transmission of an active transcriptional structure.EMBO J. 1994 Jul 1;13(13):3065-76. doi: 10.1002/j.1460-2075.1994.tb06605.x. EMBO J. 1994. PMID: 8039501 Free PMC article.
-
Complexity of the erythroid transcription factor NF-E2 as revealed by gene targeting of the mouse p18 NF-E2 locus.Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3514-8. doi: 10.1073/pnas.93.8.3514. Proc Natl Acad Sci U S A. 1996. PMID: 8622968 Free PMC article.
-
The Roles of NRF2 in Modulating Cellular Iron Homeostasis.Antioxid Redox Signal. 2018 Dec 10;29(17):1756-1773. doi: 10.1089/ars.2017.7176. Epub 2017 Sep 21. Antioxid Redox Signal. 2018. PMID: 28793787 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
