Endosome-lysosome fusion
- PMID: 21118098
- DOI: 10.1042/BST0381413
Endosome-lysosome fusion
Abstract
The delivery of endocytosed cargo to lysosomes occurs through kissing and direct fusion of late endosomes/MVBs (multivesicular bodies) and lysosomes. Live-cell and electron microscopy experiments together with cell-free assays have allowed us to describe the characteristics of the delivery process and determine the core protein machinery required for fusion. The ESCRT (endosomal sorting complex required for transport) machinery is required for MVB biogenesis. The HOPS (homotypic fusion and vacuole protein sorting) complex is required for endosome-lysosome tethering and a trans-SNARE (soluble N-ethylmaleimide-sensitive factor-attachment protein receptor) complex including the R-SNARE VAMP7 (vesicle-associated membrane protein 7) mediates endosome-lysosome membrane fusion. Protein-binding partners of VAMP7 including the clathrin adaptors AP-3 (adaptor protein 3) and Hrb (HIV Rev-binding protein) are required for its correct intracellular localization and function. Overall, co-ordination of the activities of ESCRT, HOPS and SNARE complexes are required for efficient delivery of endocytosed macromolecules to lysosomes. Endosome-lysosome fusion results in a hybrid organelle from which lysosomes are re-formed. Defects in fusion and/or lysosome reformation occur in a number of lysosome storage diseases.
Similar articles
-
The delivery of endocytosed cargo to lysosomes.Biochem Soc Trans. 2009 Oct;37(Pt 5):1019-21. doi: 10.1042/BST0371019. Biochem Soc Trans. 2009. PMID: 19754443 Review.
-
Ultrastructural analysis of ESCRT proteins suggests a role for endosome-associated tubular-vesicular membranes in ESCRT function.Traffic. 2006 Nov;7(11):1551-66. doi: 10.1111/j.1600-0854.2006.00489.x. Epub 2006 Oct 2. Traffic. 2006. PMID: 17014699
-
Distinct features of multivesicular body-lysosome fusion revealed by a new cell-free content-mixing assay.Traffic. 2018 Feb;19(2):138-149. doi: 10.1111/tra.12543. Epub 2017 Dec 10. Traffic. 2018. PMID: 29135058
-
The HOPS proteins hVps41 and hVps39 are required for homotypic and heterotypic late endosome fusion.Traffic. 2013 Feb;14(2):219-32. doi: 10.1111/tra.12027. Epub 2012 Dec 12. Traffic. 2013. PMID: 23167963
-
Multivesicular bodies and multivesicular endosomes: the "ins and outs" of endosomal traffic.Sci STKE. 2002 Jul 16;2002(141):pe32. doi: 10.1126/stke.2002.141.pe32. Sci STKE. 2002. PMID: 12122203 Review.
Cited by
-
The role of exosomes in stroke.Mol Biol Rep. 2020 Aug;47(8):6217-6228. doi: 10.1007/s11033-020-05569-2. Epub 2020 Jun 8. Mol Biol Rep. 2020. PMID: 32514999 Review.
-
Cooperation and Interplay between EGFR Signalling and Extracellular Vesicle Biogenesis in Cancer.Cells. 2020 Dec 8;9(12):2639. doi: 10.3390/cells9122639. Cells. 2020. PMID: 33302515 Free PMC article. Review.
-
Preparing the lethal hit: interplay between exo- and endocytic pathways in cytotoxic T lymphocytes.Cell Mol Life Sci. 2017 Feb;74(3):399-408. doi: 10.1007/s00018-016-2350-7. Epub 2016 Sep 1. Cell Mol Life Sci. 2017. PMID: 27585956 Free PMC article. Review.
-
Key roles of autophagosome/endosome maturation mediated by Syntaxin17 in methamphetamine-induced neuronal damage in mice.Mol Med. 2024 Jan 3;30(1):4. doi: 10.1186/s10020-023-00765-9. Mol Med. 2024. PMID: 38172666 Free PMC article.
-
Lysosome biogenesis: Regulation and functions.J Cell Biol. 2021 Jun 7;220(6):e202102001. doi: 10.1083/jcb.202102001. Epub 2021 May 5. J Cell Biol. 2021. PMID: 33950241 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
