doi: 10.1016/0378-1119(90)90288-3.
Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells
Affiliations
- PMID: 2108907
- DOI: 10.1016/0378-1119(90)90288-3
Item in Clipboard
Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells
Gene.
.
Abstract
Gene expression in heterologous species is a powerful tool for the cloning and characterization of genes. Here, we present a family of expression shuttle vectors which work in the budding yeast, Saccharomyces cerevisiae, and also in mammalian cells. The quantity of product expressed by the gene under study can be modulated in yeast by virtue of the control over plasmid copy number and culture conditions.
Similar articles
-
A family of multifunctional thiamine-repressible expression vectors for fission yeast.Yeast. 2000 Jun 30;16(9):861-72. doi: 10.1002/1097-0061(20000630)16:9<861::AID-YEA577>3.0.CO;2-9. Yeast. 2000. PMID: 10861909
-
An inducible expression vector for both fission and budding yeast.Gene. 1990 Feb 14;86(2):257-61. doi: 10.1016/0378-1119(90)90287-2. Gene. 1990. PMID: 2108906
-
A series of yeast shuttle vectors for expression of cDNAs and other DNA sequences.Yeast. 1993 Dec;9(12):1299-308. doi: 10.1002/yea.320091203. Yeast. 1993. PMID: 8154181
-
Saccharomyces cerevisiae Shuttle vectors.Yeast. 2017 May;34(5):205-221. doi: 10.1002/yea.3228. Epub 2017 Feb 21. Yeast. 2017. PMID: 28072905 Review.
-
Propagation and expression of cloned genes in yeast: 2-microns circle-based vectors.Methods Enzymol. 1990;185:234-79. doi: 10.1016/0076-6879(90)85024-i. Methods Enzymol. 1990. PMID: 2199781 Review. No abstract available.
Cited by
-
In Xenopus laevis, the product of a developmentally regulated mRNA is structurally and functionally homologous to a Saccharomyces cerevisiae protein involved in translation fidelity.Mol Cell Biol. 1993 May;13(5):2815-21. doi: 10.1128/mcb.13.5.2815-2821.1993. Mol Cell Biol. 1993. PMID: 8474443 Free PMC article.
-
ami1, an orthologue of the Aspergillus nidulans apsA gene, is involved in nuclear migration events throughout the life cycle of Podospora anserina.Genetics. 2000 Jun;155(2):633-46. doi: 10.1093/genetics/155.2.633. Genetics. 2000. PMID: 10835387 Free PMC article.
-
Versatile vectors to study recoding: conservation of rules between yeast and mammalian cells.Nucleic Acids Res. 1995 May 11;23(9):1557-60. doi: 10.1093/nar/23.9.1557. Nucleic Acids Res. 1995. PMID: 7784210 Free PMC article.
-
Two subclasses of guanine exchange factor (GEF) domains revealed by comparison of activities of chimeric genes constructed from CDC25, SDC25 and BUD5 in Saccharomyces cerevisiae.Mol Gen Genet. 1994 Oct 28;245(2):167-76. doi: 10.1007/BF00283264. Mol Gen Genet. 1994. PMID: 7816024
-
Two copies of mthmg1, encoding a novel mitochondrial HMG-like protein, delay accumulation of mitochondrial DNA deletions in Podospora anserina.Eukaryot Cell. 2002 Aug;1(4):503-13. doi: 10.1128/EC.1.4.503-513.2002. Eukaryot Cell. 2002. PMID: 12455999 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
