doi: 10.1515/bchm3.1990.371.2.929.
Non-radioactive labeling and detection of nucleic acids. II. Optimization of the digoxigenin system
Affiliations
- PMID: 2076200
- DOI: 10.1515/bchm3.1990.371.2.929
Item in Clipboard
Non-radioactive labeling and detection of nucleic acids. II. Optimization of the digoxigenin system
Biol Chem Hoppe Seyler.
1990 Oct.
Abstract
The random-primed DNA labeling technique was modified for the incorporation of digoxigenin into DNA as basic component of the digoxigenin-based non-radioactive DNA labeling and detection system. Digoxigenin molecules act as reporter groups for highly sensitive DNA detection by a digoxigenin-specific antibody:alkaline phosphatase-conjugate-catalysed color reaction. The parameters affecting the individual reaction steps of the digoxigenin labeling, hybridization and detection reactions were optimized to maximal sensitivity and specificity.
Similar articles
-
Non-radioactive labeling and detection of nucleic acids. I. A novel DNA labeling and detection system based on digoxigenin: anti-digoxigenin ELISA principle (digoxigenin system).Biol Chem Hoppe Seyler. 1990 Oct;371(10):917-27. doi: 10.1515/bchm3.1990.371.2.917. Biol Chem Hoppe Seyler. 1990. PMID: 2076199
-
Non-radioactive labeling and detection of nucleic acids. III. Applications of the digoxigenin system.Biol Chem Hoppe Seyler. 1990 Oct;371(10):939-51. doi: 10.1515/bchm3.1990.371.2.939. Biol Chem Hoppe Seyler. 1990. PMID: 1963785
-
Sensitive chemiluminescent detection of digoxigenin-labeled nucleic acids: a fast and simple protocol and its applications.Biotechniques. 1992 Jan;12(1):104-13. Biotechniques. 1992. PMID: 1370901
-
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic acids--an overview.Cell Mol Biol (Noisy-le-grand). 1995 Nov;41(7):883-905. Cell Mol Biol (Noisy-le-grand). 1995. PMID: 8595368 Review.
-
The digoxigenin:anti-digoxigenin (DIG) technology--a survey on the concept and realization of a novel bioanalytical indicator system.Mol Cell Probes. 1991 Jun;5(3):161-205. doi: 10.1016/0890-8508(91)90041-h. Mol Cell Probes. 1991. PMID: 1870582 Review.
Cited by
-
Bispecific digoxigenin-binding antibodies for targeted payload delivery.Proc Natl Acad Sci U S A. 2011 May 17;108(20):8194-9. doi: 10.1073/pnas.1018565108. Epub 2011 May 2. Proc Natl Acad Sci U S A. 2011. PMID: 21536919 Free PMC article.
-
PCR for detection of Shigella spp. in mayonnaise.Appl Environ Microbiol. 1998 Apr;64(4):1242-5. doi: 10.1128/AEM.64.4.1242-1245.1998. Appl Environ Microbiol. 1998. PMID: 9546158 Free PMC article.
-
Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA.Nucleic Acids Res. 2003 May 15;31(10):2636-46. doi: 10.1093/nar/gkg371. Nucleic Acids Res. 2003. PMID: 12736314 Free PMC article.
-
Fluorescent Citrine-IgG fusion proteins produced in mammalian cells.MAbs. 2010 Nov-Dec;2(6):648-61. doi: 10.4161/mabs.2.6.13179. Epub 2010 Nov 1. MAbs. 2010. PMID: 20724830 Free PMC article.
-
Endosymbiotic microflora of the vestimentiferan tubeworm ( Lamellibrachia sp.) from a bathyal cold seep.Mar Biotechnol (NY). 2003 Nov-Dec;5(6):593-603. doi: 10.1007/s10126-002-0117-7. Epub 2003 Sep 29. Mar Biotechnol (NY). 2003. PMID: 14508655
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources