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. 2010 Sep 15;102(4):348-53.
doi: 10.1002/jso.21649.

Preoperative dietary restriction reduces hepatic tumor load by reduced E-selectin-mediated adhesion in mice

Tessa M van Ginhoven  1 , Jan Willem van den Berg, Willem A Dik, Jan N M Ijzermans, Ron W F de Bruin
Affiliations

Preoperative dietary restriction reduces hepatic tumor load by reduced E-selectin-mediated adhesion in mice

Tessa M van Ginhoven et al. J Surg Oncol. .

Abstract

Background: Inflammatory responses facilitate metastasis by increasing expression of adhesion molecules. Dietary restriction (30% reduction in daily calorie intake) reduces the expression of adhesion molecules and protects against surgically induced inflammation. DR might therefore beneficially interfere with surgery-induced inflammation and subsequent adhesion of circulating tumor cells.

Methods: BALB/c mice were subjected to 2 weeks dietary restriction prior to inoculation with tumor cells. Intra-splenic injection of 5.0 x 10(4) C26-colon carcinoma cells was followed by splenectomy. Hepatic tumor load was scored after 10 days as a percentage (tumor surface/total liver surface) on H&E stained sections. Liver mRNA expression of adhesion molecules was determined and the effect of serum from dietary restriction mice on in vitro tumor growth and adhesion capacity was assessed.

Results: Preoperative dietary restriction significantly reduced mRNA expression levels of E-selectin (P = 0.0087) and hepatic tumor load (P = 0.036). Dietary restriction serum did not affect in vitro cell growth but reduced in vitro adhesion of C26 cells to endothelial cells (P = 0.0043).

Conclusions: Preoperative dietary restriction reduces hepatic tumor load after injection with tumor cells. Reduced adhesion to endothelial cells and reduced mRNA expression of E-selectin suggest that dietary restriction reduces tumor load by lowering the adhesion of circulating tumor cells to hepatic vascular endothelium.

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