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. 2010 Jul;9(7):572-8.
doi: 10.1038/nmat2784. Epub 2010 Jun 20.

Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines

Tomonori Nochi  1 , Yoshikazu Yuki, Haruko Takahashi, Shin-ichi Sawada, Mio Mejima, Tomoko Kohda, Norihiro Harada, Il Gyu Kong, Ayuko Sato, Nobuhiro Kataoka, Daisuke Tokuhara, Shiho Kurokawa, Yuko Takahashi, Hideo Tsukada, Shunji Kozaki, Kazunari Akiyoshi, Hiroshi Kiyono
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Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines

Tomonori Nochi et al. Nat Mater. 2010 Jul.

Erratum in

  • Nat Mater. 2010 Aug;9(8):685

Abstract

Nanotechnology is an innovative method of freely controlling nanometre-sized materials. Recent outbreaks of mucosal infectious diseases have increased the demands for development of mucosal vaccines because they induce both systemic and mucosal antigen-specific immune responses. Here we developed an intranasal vaccine-delivery system with a nanometre-sized hydrogel ('nanogel') consisting of a cationic type of cholesteryl-group-bearing pullulan (cCHP). A non-toxic subunit fragment of Clostridium botulinum type-A neurotoxin BoHc/A administered intranasally with cCHP nanogel (cCHP-BoHc/A) continuously adhered to the nasal epithelium and was effectively taken up by mucosal dendritic cells after its release from the cCHP nanogel. Vigorous botulinum-neurotoxin-A-neutralizing serum IgG and secretory IgA antibody responses were induced without co-administration of mucosal adjuvant. Importantly, intranasally administered cCHP-BoHc/A did not accumulate in the olfactory bulbs or brain. Moreover, intranasally immunized tetanus toxoid with cCHP nanogel induced strong tetanus-toxoid-specific systemic and mucosal immune responses. These results indicate that cCHP nanogel can be used as a universal protein-based antigen-delivery vehicle for adjuvant-free intranasal vaccination.

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References

    1. Biotechnol Bioeng. 2001 Jul 5;74(1):62-9 - PubMed
    1. Infect Immun. 2007 Jun;75(6):3043-54 - PubMed
    1. Nat Rev Immunol. 2004 Sep;4(9):699-710 - PubMed
    1. FEBS Lett. 2003 Oct 23;553(3):271-6 - PubMed
    1. Nat Med. 2005 Apr;11(4 Suppl):S45-53 - PubMed

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