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Review
. 2010 Jun;22(3):305-13.
doi: 10.1016/j.ceb.2010.04.005. Epub 2010 May 11.

3D shortcuts to gene regulation

Ofir Hakim  1 , Myong-Hee Sung, Gordon L Hager
Affiliations
Review

3D shortcuts to gene regulation

Ofir Hakim et al. Curr Opin Cell Biol. 2010 Jun.

Abstract

Recent technologies have allowed high-resolution genome-wide binding profiles of numerous transcription factors and other proteins. A widespread observation has emerged from studies in diverse mammalian systems: most binding events are located at great distances from gene promoters. It is becoming apparent that the traditional one-dimensional view of gene regulation via the proximal cis regulatory elements is over-simplified. True proximity and functional relevance can be revealed by studying the three-dimensional structure of the genome packaged inside the nucleus. Thus the spatial architecture of the genome has attracted a lot of interest and has intensified its significance in modern cell biology. Here we discuss current methods, concepts, and controversies in this rapidly evolving field.

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Figures

Figure 1
Figure 1
Microarray profile of long range interactions by 4C. (A) Long-range contacts of the bait across the same chromosome (top profile) and with loci on another chromosome (bottom profile). The peaks represent long-range contacts and their magnitudes indicate the interaction frequency. The dominant peak cluster centered at the bait reflects the high interaction frequency of the locus with the nearby sequences within a few million bp distance. Most of the reported distant regulatory elements are found within this region (panel B). (B) Finer-scale spatial interactions within the bait region. Regulatory sites (ellipses) are in long-range contacts (arrows) with other regulatory sites and genes (gray rectangle), generating a complex network of direct and indirect contacts. (C) The chromosomes are folded in the interphase nucleus into chromosome territories. Chromosomal loci are non-randomly engaged in long-range contacts with other distant loci from the same chromosomes and with loci from other chromosomes creating spatial micro-environments.
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