Osteogenic differentiation of bone marrow mesenchymal stem cells by adenovirus-mediated expression of leptin
- PMID: 20433876
- DOI: 10.1016/j.regpep.2010.04.006
Osteogenic differentiation of bone marrow mesenchymal stem cells by adenovirus-mediated expression of leptin
Abstract
Previous studies demonstrate that leptin has an osteogenic differentiation effect on bone marrow mesenchymal stem cells (MSCs). However, the effect of adenovirus-mediated leptin on MSCs differentiation has not been reported. To explore the mechanism, we constructed a recombinant adenoviral vector Ad-leptin and transfected propagated MSCs in vitro. The effects of Ad-leptin on MSCs growth and osteogenic differentiation were examined. The results showed that Ad-leptin inhibited the transfected MSCs growth significantly, and up-regulated osteocalcin expression and alkaline phosphatase activity. The expression of Cbfalpha1 and Cbfbeta which were the key factors in osteogenic differentiation was also up-regulated. All the findings suggest that genetic engineering of MSCs to express leptin gene may have potential application in the treatment of several genetic diseases and bone reconstruction.
Copyright (c) 2010 Elsevier B.V. All rights reserved.
Similar articles
-
Comparison of multipotent differentiation potentials of murine primary bone marrow stromal cells and mesenchymal stem cell line C3H10T1/2.Calcif Tissue Int. 2009 Jan;84(1):56-64. doi: 10.1007/s00223-008-9189-3. Epub 2008 Dec 4. Calcif Tissue Int. 2009. PMID: 19052794
-
Cbfb enhances the osteogenic differentiation of both human and mouse mesenchymal stem cells induced by Cbfa-1 via reducing its ubiquitination-mediated degradation.Stem Cells. 2007 Jun;25(6):1462-8. doi: 10.1634/stemcells.2006-0391. Epub 2007 Mar 22. Stem Cells. 2007. PMID: 17379770
-
Adenovirus mediated BMP-13 gene transfer induces chondrogenic differentiation of murine mesenchymal progenitor cells.J Bone Miner Res. 2004 Jan;19(1):111-22. doi: 10.1359/jbmr.2004.19.1.111. J Bone Miner Res. 2004. PMID: 14753743
-
Gene-mediated osteogenic differentiation of stem cells by bone morphogenetic proteins-2 or -6.J Orthop Res. 2006 Jun;24(6):1279-91. doi: 10.1002/jor.20068. J Orthop Res. 2006. PMID: 16649180
-
[Recent advances in research on mesenchymal stem cells].Nihon Yakurigaku Zasshi. 2014 Dec;144(6):305. doi: 10.1254/fpj.144.305. Nihon Yakurigaku Zasshi. 2014. PMID: 25492367 Review. Japanese. No abstract available.
Cited by
-
Leptin Receptor Metabolism Disorder in Primary Chondrocytes from Adolescent Idiopathic Scoliosis Girls.Int J Mol Sci. 2016 Jul 20;17(7):1160. doi: 10.3390/ijms17071160. Int J Mol Sci. 2016. PMID: 27447624 Free PMC article.
-
Fate decision of mesenchymal stem cells: adipocytes or osteoblasts?Cell Death Differ. 2016 Jul;23(7):1128-39. doi: 10.1038/cdd.2015.168. Epub 2016 Feb 12. Cell Death Differ. 2016. PMID: 26868907 Free PMC article. Review.
-
Microenvironmental factors that regulate mesenchymal stem cells: lessons learned from the study of heterotopic ossification.Histol Histopathol. 2017 Oct;32(10):977-985. doi: 10.14670/HH-11-890. Epub 2017 Mar 22. Histol Histopathol. 2017. PMID: 28328009 Free PMC article. Review.
-
Adipokines at the crossroads of obesity and mesenchymal stem cell therapy.Exp Mol Med. 2023 Feb;55(2):313-324. doi: 10.1038/s12276-023-00940-2. Epub 2023 Feb 7. Exp Mol Med. 2023. PMID: 36750692 Free PMC article. Review.
-
Altered expression of leptin and leptin receptor in the development of immune-mediated aplastic anemia in mice.Exp Ther Med. 2019 Aug;18(2):1047-1056. doi: 10.3892/etm.2019.7660. Epub 2019 Jun 11. Exp Ther Med. 2019. PMID: 31316601 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
