Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1991 Mar 1;88(5):1968-72.
doi: 10.1073/pnas.88.5.1968.

A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast

D M Becker  1 , J D Fikes, L Guarente
Affiliations
Comparative Study

A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast

D M Becker et al. Proc Natl Acad Sci U S A. .

Abstract

We constructed a comprehensive cDNA library from HeLa cell mRNA in a vector that directs expression of the cDNA in Saccharomyces cerevisiae. We used this library to clone the human counterpart of the Sa. cerevisiae CCAAT-binding transcription factor, Hap2, by functional complementation of a hap2 mutation. The cDNA encoding the human Hap2 homolog encodes a protein of 257 amino acids that has a 62-amino acid carboxyl-terminal region 73% identical to the essential core region of Hap2. The amino terminus of the protein is highly enriched in glutamine residues, reminiscent of transcriptional activation domains of several other mammalian transcription factors. Analysis of human Hap2 expression reveals three major transcripts: a 4.1-kilobase species found in all cell types examined, a 7.0-kilobase species specific to B lymphocytes, and a 1.6-kilobase species that is expressed preferentially in HeLa cells and that likely corresponds to our cDNA clone. Thus, the human Hap2 homolog and related factors may play both a constitutive and cell type-specific role in gene expression. The general approach of cloning by complementation should allow the isolation of many human genes for which corresponding yeast mutations exist.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Annu Rev Cell Biol. 1989;5:153-80 - PubMed
    1. Cell. 1988 Dec 23;55(6):989-1003 - PubMed
    1. Proc Natl Acad Sci U S A. 1990 Apr;87(8):2916-20 - PubMed
    1. EMBO J. 1990 May;9(5):1543-9 - PubMed
    1. EMBO J. 1990 May;9(5):1625-34 - PubMed

Publication types

Substances