Puerarin inhibits acid-sensing ion channels and protects against neuron death induced by acidosis
- PMID: 19918715
- DOI: 10.1055/s-0029-1240583
Puerarin inhibits acid-sensing ion channels and protects against neuron death induced by acidosis
Abstract
Puerarin, the main isoflavone found in the root of the wild leguminous creeper Pueraria lobata (Willd.) Ohwi, is known to protect the brain from ischemia-induced injuries. However, its effect on acidosis, a byproduct of brain ischemia and one factor promoting cell death, remains unknown. The purpose of this study was to investigate the effects of puerarin on acid-sensing ion channels (ASICs), which are reported to be activated by extracellular acidosis. Using the MTT assay and Hoechst/PI staining, we showed that puerarin could prevent hippocampal cell death during extracellular low pH. Data from whole-cell patch clamp recordings demonstrated that puerarin decreased the amplitude of ASIC currents and accelerated the desensitization of ASICs. Puerarin also reduced the amplitude of homomeric ASIC1a channels in CHO cells expressing pECFP-ASIC1a. Collectively, our study demonstrated that puerarin prevented hippocampal cells from acidosis-induced death via ASIC blockage, providing a mechanical insight into the neuroprotective effects of puerarin during brain ischemia.
Georg Thieme Verlag KG Stuttgart.New York.
Similar articles
-
ASICs aggravate acidosis-induced injuries during ischemic reperfusion.Neurosci Lett. 2010 Jul 19;479(1):63-8. doi: 10.1016/j.neulet.2010.05.029. Epub 2010 May 15. Neurosci Lett. 2010. PMID: 20478356
-
Subunit-dependent cadmium and nickel inhibition of acid-sensing ion channels.Dev Neurobiol. 2007 Jan;67(1):97-107. doi: 10.1002/dneu.20338. Dev Neurobiol. 2007. PMID: 17443775
-
Properties of the proton-evoked currents and their modulation by Ca2+ and Zn2+ in the acutely dissociated hippocampus CA1 neurons.Brain Res. 2004 Aug 13;1017(1-2):197-207. doi: 10.1016/j.brainres.2004.05.046. Brain Res. 2004. PMID: 15261115
-
Acid sensing ion channels--novel therapeutic targets for ischemic brain injury.Front Biosci. 2007 Jan 1;12:1376-86. doi: 10.2741/2154. Front Biosci. 2007. PMID: 17127388 Review.
-
Management of Diabetes Mellitus with Puerarin, a Natural Isoflavone From Pueraria lobata.Am J Chin Med. 2018;46(8):1771-1789. doi: 10.1142/S0192415X18500891. Epub 2018 Dec 10. Am J Chin Med. 2018. PMID: 30525896 Review.
Cited by
-
Investigating the Potential Therapeutic Mechanisms of Puerarin in Neurological Diseases.Mol Neurobiol. 2024 Dec;61(12):10747-10769. doi: 10.1007/s12035-024-04222-4. Epub 2024 May 23. Mol Neurobiol. 2024. PMID: 38780722 Review.
-
Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils.Chin J Integr Med. 2012 Apr;18(4):283-7. doi: 10.1007/s11655-012-1054-6. Epub 2012 Mar 30. Chin J Integr Med. 2012. PMID: 22457139
-
Acid-sensing ion channel 3 deficiency increases inflammation but decreases pain behavior in murine arthritis.Arthritis Rheum. 2013 May;65(5):1194-202. doi: 10.1002/art.37862. Arthritis Rheum. 2013. PMID: 23335302 Free PMC article.
-
Acid-sensing ion channels in pain and disease.Nat Rev Neurosci. 2013 Jul;14(7):461-71. doi: 10.1038/nrn3529. Nat Rev Neurosci. 2013. PMID: 23783197 Free PMC article. Review.
-
Ionic regulation of cell volume changes and cell death after ischemic stroke.Transl Stroke Res. 2014 Feb;5(1):17-27. doi: 10.1007/s12975-013-0314-x. Epub 2013 Dec 7. Transl Stroke Res. 2014. PMID: 24323733 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
