Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Feb;11(2):773-84.
doi: 10.1128/mcb.11.2.773-784.1991.

Extracellular signals that regulate liver transcription factors during hepatic differentiation in vitro

J K Liu  1 , C M DiPersio, K S Zaret
Affiliations

Extracellular signals that regulate liver transcription factors during hepatic differentiation in vitro

J K Liu et al. Mol Cell Biol. 1991 Feb.

Abstract

A complex cell culture environment has been shown to maintain the differentiated state of hepatocytes, yet the mechanisms by which environmental cues selectively maintain liver-specific gene transcription have been unknown. In this paper we show that the hepatic environment regulates the activities of at least three liver-enriched transcription factors, eE-TF, eG-TF/HNF3, and eH-TF, that activate the mouse serum albumin enhancer. eE-TF is a heat-stable factor that has a DNA-binding specificity similar to that of the liver transcription factor C/EBP, but is a distinct protein. eG-TF/HNF3 contributes to the liver-specific transcription of several other serum protein genes. eH-TF binds to a TGTTTGC sequence that occurs at regulatory sites of the albumin promoter, the hepatitis B virus enhancer, and other hepatic genes. eE-TF, eG-TF/HNF3, and eH-TF are regulated by different combinations of the following cell culture conditions: a hormonally defined serum-free medium; an extracellular matrix gel; and a transformation-competent simian virus 40 large T antigen. We propose a regulatory network model to explain how cues from the cell lineage and the extracellular environment coordinately help maintain the activities of transcription factors involved in hepatocyte differentiation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Cell Biol. 1983 Sep;3(9):1552-61 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jan;84(1):136-40 - PubMed
    1. Hepatology. 1984 May-Jun;4(3):548-59 - PubMed
    1. Science. 1987 Apr 3;236(4797):55-63 - PubMed
    1. Science. 1987 Jun 5;236(4806):1237-45 - PubMed

Publication types

MeSH terms

LinkOut - more resources