Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb;127(2):207-21.
doi: 10.1007/s00439-009-0763-1. Epub 2009 Nov 10.

Rubella vaccine-induced cellular immunity: evidence of associations with polymorphisms in the Toll-like, vitamin A and D receptors, and innate immune response genes

Inna G Ovsyannikova  1 , Neelam Dhiman, Iana H Haralambieva, Robert A Vierkant, Megan M O'Byrne, Robert M Jacobson, Gregory A Poland
Affiliations

Rubella vaccine-induced cellular immunity: evidence of associations with polymorphisms in the Toll-like, vitamin A and D receptors, and innate immune response genes

Inna G Ovsyannikova et al. Hum Genet. 2010 Feb.

Abstract

Toll-like, vitamin A and D receptors and other innate proteins participate in various immune functions. We determined whether innate gene-sequence variations are associated with rubella vaccine-induced cytokine immune responses. We genotyped 714 healthy children (11-19 years of age) after two doses of rubella-containing vaccine for 148 candidate SNP markers. Rubella virus-induced cytokines were measured by ELISA. Twenty-two significant associations (range of P values 0.002-0.048) were found between SNPs in the vitamin A receptor family (RARA, RARB, TOP2B and RARG), vitamin D receptor and downstream mediator of vitamin D signaling (RXRA) genes and rubella virus-specific (IFN-gamma, IL-2, IL-10, TNF-alpha, and GM-CSF) cytokine immune responses. A TLR3 gene promoter region SNP (rs5743305, -8441A > T) was associated with rubella-specific GM-CSF secretion. Importantly, SNPs in the TRIM5 gene coding regions, rs3740996 (His43Tyr) and rs10838525 (Gln136Arg), were associated with an allele dose-related secretion of rubella virus-specific TNF-alpha and IL-2/GM-CSF, respectively, and have been previously shown to have functional consequences regarding the antiviral activity and susceptibility to HIV-1 infection. We identified associations between individual SNPs and haplotypes in, or involving, the RIG-I (DDX58) gene and rubella-specific TNF-alpha secretion. This is the first paper to present evidence that polymorphisms in the TLR, vitamin A, vitamin D receptor, and innate immunity genes can influence adaptive cytokine responses to rubella vaccination.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
The linkage disequilibrium output for DDX58 SNPs from Haploview. Haplotype block structure of the DDX58 (RIG-I) gene region in the study cohort. Three intronic (rs592515 and rs6476363 and rs3824456, r2 ≥ 0.16) and three 5′UTR/intronic (rs3739674 and rs10813829 and rs4633144, r2 ≥ 0.92) SNPs from the DDX58 gene were associated with TNF-α secretion. The LD block structure was analyzed using Haploview software, version 3.32. The r2 color scheme is: white (r2 = 0), shades of gray (0 < r2 < 1), black (r2 = 1)
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Arai H, Miyamoto KI, Yoshida M, Yamamoto H, Taketani Y, Morita K, Kubota M, Yoshida S, Ikeda M, Watabe F, Kanemasa Y, Takeda E. The polymorphism in the caudal-related homeodomain protein Cdx-2 binding element in the human vitamin D receptor gene. J Bone Miner Res. 2001;16:1256–1264. - PubMed
    1. Bahl R, Bhandari N, Kant S, Molbak K, Ostergaard E, Bhan MK. Effect of vitamin A administered at Expanded Program on Immunization contacts on antibody response to oral polio vaccine. Eur J Clin Nutr. 2002;56:321–325. - PubMed
    1. Barrett JC, Fry B, Maller J, Daly MJ. Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics. 2005;21:263–265. - PubMed
    1. Benn CS, Balde A, George E, Kidd M, Whittle H, Lisse IM, Aaby P. Effect of vitamin A supplementation on measles-specific antibody levels in Guinea-Bissau. Lancet. 2002;359:1313–1314. - PubMed
    1. Biacchesi S, LeBerre M, Lamoureux A, Louise Y, Lauret E, Boudinot P, Bremont M. Mitochondrial antiviral signaling protein plays a major role in induction of the fish innate immune response against RNA and DNA Viruses. J Virol. 2009;83:7815–7827. - PMC - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources