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. 2009 Nov;94(11):1563-8.
doi: 10.3324/haematol.2009.008474.

Strong lymphoid nuclear expression of SOX11 transcription factor defines lymphoblastic neoplasms, mantle cell lymphoma and Burkitt's lymphoma

Michael Dictor  1 , Sara Ek, Maria Sundberg, Janina Warenholt, Czabafy György, Sandra Sernbo, Elin Gustavsson, Waleed Abu-Alsoud, Torkel Wadström, Carl Borrebaeck
Affiliations

Strong lymphoid nuclear expression of SOX11 transcription factor defines lymphoblastic neoplasms, mantle cell lymphoma and Burkitt's lymphoma

Michael Dictor et al. Haematologica. 2009 Nov.

Abstract

Background: We surveyed lymphomas to determine the range of expression of the mantle cell lymphoma-associated SOX11 transcription factor and its relation to cyclin D1.

Design and methods: On hundred and seventy-two specimens were immunostained for the SOX11 N and C termini. Cyclin D1 was detected by immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction; in situ hybridization for t(11;14) was applied when needed.

Results: Nuclear SOX11 was strongly expressed in most B and T-lymphoblastic leukemia/lymphomas and half of childhood Burkitt's lymphomas, but only weakly expressed in some hairy cell leukemias. Chronic lymphocytic leukemia/lymphoma, marginal zone, follicular and diffuse large B-cell lymphomas were negative for SOX11, as were all cases of intermediate Burkitt's lymphomas/diffuse large B-cell lymphoma, myeloma, Hodgkin's lymphomas and mature T-cell and NK/T-cell lymphomas.

Conclusions: In addition to mantle cell lymphoma, SOX11 is strongly expressed only in lymphoblastic malignancies and Burkitt's lymphomas. Its expression is independent of cyclin D1 (except for weak expression in hairy cell leukemias) and unlikely to be due to translocations in lymphoid neoplasia.

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Figures

Figure 1.
Figure 1.
(A) A Western blot of proteins extracted from two MCL cell lines showing bands of approximately 60 kDa for SOX11 using either anti-SOX11 antibody. (B) The lane labeled SOX11 denotes Granta-519 cell extract after knock-down with specific siRNA and staining with anti-SOX11C-term, which yielded no band, in contrast to the SOX11 bands noted in negative and control lanes; these lanes contain extracts after nucleofection with scrambled sequence siRNA and untransfected cells, respectively. (C) A case of MCL (MCL1) with weak nuclear signals after applying SOX11N-term showed stronger signals using SOX11C-term. Another case of MCL (MCL2) showed only cytoplasmic signals until immunoreacted with SOX11C-term, after which nuclear signals appeared (DAB with hematoxylin counterstain, Olympus BX45, magnification x125, colors corrected after acquisition with Adobe Photoshop). (D) Strong nuclear SOX11 signals after staining with anti-SOX11C-term is seen in a true Burkitt’s lymphoma. (E) Intermediate Burkitt’s lymphoma/diffuse large B-cell lymphoma shows no nuclear stain (signal is limited to cytoplasm). (F) Positive nuclear staining in lymphoblastic neoplasia is exemplified by a case of adult nodal T-cell lymphoblastic lymphoma (inset, TdT stain). (G) Signals are present in the bone marrow from a patient with B-cell acute lymphoblastic leukemia. (H) A case of childhood orbital B-cell lymphoblastic lymphoma also expresses SOX11. (I) Bone marrow in hairy cell leukemia, case 9, expressing DBA.44 (inset, upper left), CCND1 (inset, lower right) and SOX11 detected with anti-SOX11C-term (DAB with hematoxylin counterstain, magnification x125, except D, x230,).
See this image and copyright information in PMC

Comment in

  • Mantle cell lymphoma.
    Pileri SA, Falini B. Pileri SA, et al. Haematologica. 2009 Nov;94(11):1488-92. doi: 10.3324/haematol.2009.013359. Haematologica. 2009. PMID: 19880776 Free PMC article. Review.

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References

    1. Ek S, Dictor M, Jerkeman M, Jirstrom K, Borrebaeck CA. Nuclear expression of the non B-cell lineage Sox11 transcription factor identifies mantle cell lymphoma. Blood. 2008;111:800–5. - PubMed
    1. Wang X, Asplund AC, Porwit A, Flygare J, Smith CI, Christensson B, et al. The subcellular Sox11 distribution pattern identifies subsets of mantle cell lymphoma: correlation to overall survival. Br J Haematol. 2008;143:248–52. - PubMed
    1. Troussard X, Avet-Loiseau H, Macro M, Mellerin MP, Malet M, Roussel M, et al. Cyclin D1 expression in patients with multiple myeloma. Hematol J. 2000;1:181–5. - PubMed
    1. Bosch F, Campo E, Jares P, Pittaluga S, Munoz J, Nayach I, et al. Increased expression of the PRAD-1/CCND1 gene in hairy cell leukaemia. Br J Haematol. 1995;91:1025–30. - PubMed
    1. de Boer CJ, Kluin-Nelemans JC, Dreef E, Kester MG, Kluin PM, Schuuring E, et al. Involvement of the CCND1 gene in hairy cell leukemia. Ann Oncol. 1996;7:251–6. - PubMed

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