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. 2009 Oct 6;101(7):1194-8.
doi: 10.1038/sj.bjc.6605290.

Salivary analysis of oral cancer biomarkers

T Shpitzer  1 , Y Hamzany, G Bahar, R Feinmesser, D Savulescu, I Borovoi, M Gavish, R M Nagler
Affiliations

Salivary analysis of oral cancer biomarkers

T Shpitzer et al. Br J Cancer. .

Abstract

Background: Oral cancer is a common and lethal malignancy. Direct contact between saliva and the oral cancer lesion makes measurement of tumour markers in saliva an attractive alternative to serum testing.

Methods: We tested 19 tongue cancer patients, measuring the levels of 8 salivary markers related to oxidative stress, DNA repair, carcinogenesis, metastasis and cellular proliferation and death.

Results: Five markers increased in cancer patients by 39-246%: carbonyls, lactate dehydrogenase, metalloproteinase-9 (MMP-9), Ki67 and Cyclin D1 (CycD1) (P< or =0.01). Three markers decreased by 16-29%: 8-oxoguanine DNA glycosylase, phosphorylated-Src and mammary serine protease inhibitor (Maspin) (P< or =0.01). Increase in salivary carbonyls was profound (by 246%, P=0.012); alterations in CycD1 (87% increase, P=0.000006) and Maspin (29% decrease, P=0.007) were especially significant. Sensitivity values of these eight analysed markers ranged from 58% to 100%; specificity values ranged from 42% to 100%. Both values were especially high for the CycD1 and Maspin markers, 100% for each value of each marker. These were also high for carbonyls, 90% and 80%, respectively, and for MMP-9, 100% and 79%, respectively.

Conclusion: The significance of each salivary alteration is discussed. As all alterations correlated with each other, they may belong to a single carcinogenetic network. Cancer-related changes in salivary tumour markers may be used as a diagnostic tool for diagnosis, prognosis and post-operative monitoring.

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Figures

Figure 1
Figure 1
Mean activity of salivary LDH (μ/l) in healthy controls (Control, n=19) and oral cancer patients (Cancer, n=19), (** P=0.002).
See this image and copyright information in PMC

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References

    1. Adjei AA (2005) Targeting multiple signal transduction pathways in lung cancer. Clin Lung Cancer 7(Suppl 1): S39–S44 - PubMed
    1. Avizienyte E, Brunton VG, Fincham VJ, Frame MC (2005) The SRC-induced mesenchymal state in late-stage colon cancer cells. Cells Tissues Organs 179: 73–80 - PubMed
    1. Bahar G, Feinmesser R, Shpitzer T, Popovtzer A, Nagler RM (2007) Salivary analysis in oral cancer patients: DNA and protein oxidation, reactive nitrogen species, and antioxidant profile. Cancer 109: 54–59 - PubMed
    1. Chen JY, Hung CC, Huang KL, Chen YT, Liu SY, Chiang WF, Chen HR, Yen CY, Wu YJ, Ko JY, Jou YS (2008) Src family kinases mediate betel quid-induced oral cancer cell motility and could be a biomarker for early invasion in oral squamous cell carcinoma. Neoplasia 10: 1393–1401 - PMC - PubMed
    1. Chen L, Sun B, Zhang S, Zhao X, He Y, Zhao S, Lin T, Li X (2009) Influence of microenvironments on microcirculation patterns and tumor invasion-related protein expression in melanoma. Oncol Rep 21: 917–923 - PubMed