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. 2009 Aug 26;4(8):e6767.
doi: 10.1371/journal.pone.0006767.

The relationship of DNA methylation with age, gender and genotype in twins and healthy controls

Marco P Boks  1 , Eske M Derks, Daniel J Weisenberger, Erik Strengman, Esther Janson, Iris E Sommer, René S Kahn, Roel A Ophoff
Affiliations

The relationship of DNA methylation with age, gender and genotype in twins and healthy controls

Marco P Boks et al. PLoS One. .

Abstract

Cytosine-5 methylation within CpG dinucleotides is a potentially important mechanism of epigenetic influence on human traits and disease. In addition to influences of age and gender, genetic control of DNA methylation levels has recently been described. We used whole blood genomic DNA in a twin set (23 MZ twin-pairs and 23 DZ twin-pairs, N = 92) as well as healthy controls (N = 96) to investigate heritability and relationship with age and gender of selected DNA methylation profiles using readily commercially available GoldenGate bead array technology. Despite the inability to detect meaningful methylation differences in the majority of CpG loci due to tissue type and locus selection issues, we found replicable significant associations of DNA methylation with age and gender. We identified associations of genetically heritable single nucleotide polymorphisms with large differences in DNA methylation levels near the polymorphism (cis effects) as well as associations with much smaller differences in DNA methylation levels elsewhere in the human genome (trans effects). Our results demonstrate the feasibility of array-based approaches in studies of DNA methylation and highlight the vast differences between individual loci. The identification of CpG loci of which DNA methylation levels are under genetic control or are related to age or gender will facilitate further studies into the role of DNA methylation and disease.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Sample plot of DNA methylation levels at probe ALOX12_P223_R by genotype of SNP rs434473.
See this image and copyright information in PMC

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References

    1. Cropley JE, Suter CM, Beckman KB, Martin DI. Germ-line epigenetic modification of the murine A vy allele by nutritional supplementation. Proc Natl Acad Sci U S A. 2006;103:17308–17312. - PMC - PubMed
    1. Weaver IC, Meaney MJ, Szyf M. Maternal care effects on the hippocampal transcriptome and anxiety-mediated behaviors in the offspring that are reversible in adulthood. Proc Natl Acad Sci U S A. 2006;103:3480–3485. - PMC - PubMed
    1. Rakyan VK, Chong S, Champ ME, Cuthbert PC, Morgan HD, et al. Transgenerational inheritance of epigenetic states at the murine Axin(Fu) allele occurs after maternal and paternal transmission. Proc Natl Acad Sci U S A. 2003;100:2538–2543. - PMC - PubMed
    1. Goldberg AD, Allis CD, Bernstein E. Epigenetics: a landscape takes shape. Cell. 2007;128:635–638. - PubMed
    1. Metivier R, Gallais R, Tiffoche C, Le PC, Jurkowska RZ, et al. Cyclical DNA methylation of a transcriptionally active promoter. Nature. 2008;452:45–50. - PubMed

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