doi: 10.1093/nar/4.5.1539.
Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide
- PMID: 19730
- PMCID: PMC343772
- DOI: 10.1093/nar/4.5.1539
Item in Clipboard
Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide
Nucleic Acids Res.
1977.
Free PMC article
Abstract
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.
Similar articles
-
RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions.Nucleic Acids Res. 1978 May;5(5):1627-37. doi: 10.1093/nar/5.5.1627. Nucleic Acids Res. 1978. PMID: 208059 Free PMC article.
-
Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea.Nucleic Acids Res. 1977 Oct;4(10):3537-55. doi: 10.1093/nar/4.10.3537. Nucleic Acids Res. 1977. PMID: 928068 Free PMC article.
-
In vitro synthesis of double stranded RNA and measurement of thermal stability: effect of base composition, formamide and ionic strength.Biochem Int. 1987 Jun;14(6):1015-22. Biochem Int. 1987. PMID: 2839182
-
Reassociation of nucleic acids in solutions containing formamide.Biochem Biophys Res Commun. 1973 Jan 4;50(1):91-7. doi: 10.1016/0006-291x(73)91068-1. Biochem Biophys Res Commun. 1973. PMID: 4567088 No abstract available.
-
Hybridization and renaturation kinetics of nucleic acids.Annu Rev Biophys Bioeng. 1976;5:337-61. doi: 10.1146/annurev.bb.05.060176.002005. Annu Rev Biophys Bioeng. 1976. PMID: 7992 Review. No abstract available.
Cited by
-
Expression of Escherichia coli dnaA and mioC genes as a function of growth rate.J Bacteriol. 1989 Aug;171(8):4272-80. doi: 10.1128/jb.171.8.4272-4280.1989. J Bacteriol. 1989. PMID: 2546917 Free PMC article.
-
Nucleotide sequence and regulated expression of a wound-inducible potato gene (wun1).Mol Gen Genet. 1989 Oct;219(1-2):81-8. doi: 10.1007/BF00261161. Mol Gen Genet. 1989. PMID: 2615766
-
Mapping early transcripts of herpes simplex virus type 1 by electron microscopy.J Virol. 1978 Jul;27(1):56-73. doi: 10.1128/JVI.27.1.56-73.1978. J Virol. 1978. PMID: 211255 Free PMC article.
-
Improvement of PCR sequencing by formamide.Nucleic Acids Res. 1991 Dec 11;19(23):6649. doi: 10.1093/nar/19.23.6649. Nucleic Acids Res. 1991. PMID: 1754407 Free PMC article.
-
Estimation of gene expression in heterocysts of Anabaena variabilis by using DNA-RNA hybridization.J Bacteriol. 1986 Sep;167(3):940-6. doi: 10.1128/jb.167.3.940-946.1986. J Bacteriol. 1986. PMID: 2427500 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
