KAP1-independent transcriptional repression of SCAN-KRAB-containing zinc finger proteins
- PMID: 19695231
- DOI: 10.1016/j.bbrc.2009.08.065
KAP1-independent transcriptional repression of SCAN-KRAB-containing zinc finger proteins
Abstract
The Krüppel-associated box-containing zinc finger gene family (KRAB-ZNF) is one of the largest gene families of transcriptional factors in the human genome. Although the functions of most of these genes remain to be determined, it is known that KRAB-mediated transcriptional repression requires a direct interaction with the KAP1 co-repressor. By mammalian one- or two-hybrid experiments in HEK293 cells, we compared transcriptional repression activities of 61 human KRAB-ZNFs. The results showed that six SCAN-KRAB-containing ZNFs are KAP1-independent transcriptional repressors whose SCAN-KRAB domain is unable to associate with KAP1 despite retaining transcriptional repression activity. Transcriptional repression activities of the SCAN-KRAB domain of KAP1-independent KRAB-ZNFs are not influenced by depletion of endogenous KAP1 levels by small interfering RNA. Although the mechanism by which KAP1-independent KRAB-ZNFs repress transcriptional activity remains to be elucidated, it appears that there may be a pathway for transcriptional repression that does not involve KAP1. These results provide new insight into the functions of the members of the KRAB-ZNF family.
Similar articles
-
The Krüppel-like zinc-finger protein ZNF224 represses aldolase A gene transcription by interacting with the KAP-1 co-repressor protein.Gene. 2005 Oct 10;359:35-43. doi: 10.1016/j.gene.2005.06.021. Gene. 2005. PMID: 16150558
-
KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading.PLoS Genet. 2010 Mar 5;6(3):e1000869. doi: 10.1371/journal.pgen.1000869. PLoS Genet. 2010. PMID: 20221260 Free PMC article.
-
A KRAB-related domain and a novel transcription repression domain in proteins encoded by SSX genes that are disrupted in human sarcomas.Oncogene. 1998 Oct 15;17(15):2013-8. doi: 10.1038/sj.onc.1202122. Oncogene. 1998. PMID: 9788446
-
Evolution of KRAB-containing zinc finger proteins and their roles in species evolution.Yi Chuan. 2016 Nov 20;38(11):971-978. doi: 10.16288/j.yczz.16-056. Yi Chuan. 2016. PMID: 27867147 Review.
-
ZFP932 suppresses cellular Hedgehog response and Patched1 transcription.Vitam Horm. 2012;88:309-32. doi: 10.1016/B978-0-12-394622-5.00014-6. Vitam Horm. 2012. PMID: 22391310 Review.
Cited by
-
ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.J Exp Med. 2021 Dec 6;218(12):e20210920. doi: 10.1084/jem.20210920. Epub 2021 Nov 11. J Exp Med. 2021. PMID: 34762123 Free PMC article.
-
The B-subdomain of the Xenopus laevis XFIN KRAB-AB domain is responsible for its weaker transcriptional repressor activity compared to human ZNF10/Kox1.PLoS One. 2014 Feb 3;9(2):e87609. doi: 10.1371/journal.pone.0087609. eCollection 2014. PLoS One. 2014. PMID: 24498343 Free PMC article.
-
KAP1 protein: an enigmatic master regulator of the genome.J Biol Chem. 2011 Jul 29;286(30):26267-76. doi: 10.1074/jbc.R111.252569. Epub 2011 Jun 7. J Biol Chem. 2011. PMID: 21652716 Free PMC article. Review.
-
The Transcriptional Repressive Activity of KRAB Zinc Finger Proteins Does Not Correlate with Their Ability to Recruit TRIM28.PLoS One. 2016 Sep 22;11(9):e0163555. doi: 10.1371/journal.pone.0163555. eCollection 2016. PLoS One. 2016. PMID: 27658112 Free PMC article.
-
Gypsy and the birth of the SCAN domain.J Virol. 2011 Nov;85(22):12043-52. doi: 10.1128/JVI.00867-11. Epub 2011 Aug 24. J Virol. 2011. PMID: 21865395 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
