Posttranslational glutamylation of alpha-tubulin
- PMID: 1967194
- DOI: 10.1126/science.1967194
Posttranslational glutamylation of alpha-tubulin
Abstract
The high degree of tubulin heterogeneity in neurons is controlled mainly at the posttranslational level. Several variants of alpha-tubulin can be posttranslationally labeled after incubation of cells with [3H]acetate or [3H]glutamate. Peptides carrying the radioactive moiety were purified by high-performance liquid chromatography. Amino acid analysis, Edman degradation sequencing, and mass spectrometric analysis of these peptides led to the characterization of a posttranslational modification consisting of the successive addition of glutamyl units on the gamma-carboxyl group of a glutamate residue (Glu445). This modification, localized within a region of alpha-tubulin that is important in the interactions of tubulin with microtubule-associated proteins and calcium, could play a role in regulating microtubule dynamics.
Similar articles
-
A combination of posttranslational modifications is responsible for the production of neuronal alpha-tubulin heterogeneity.J Cell Biochem. 1991 Jun;46(2):134-42. doi: 10.1002/jcb.240460207. J Cell Biochem. 1991. PMID: 1680872
-
Structure of the polyglutamyl side chain posttranslationally added to alpha-tubulin.J Biol Chem. 1991 Dec 5;266(34):23461-6. J Biol Chem. 1991. PMID: 1720781
-
Posttranslational modifications of the C-terminus of alpha-tubulin in adult rat brain: alpha 4 is glutamylated at two residues.Biochemistry. 1998 Oct 20;37(42):14838-44. doi: 10.1021/bi981335k. Biochemistry. 1998. PMID: 9778358
-
Mass spectrometry analysis of C-terminal posttranslational modifications of tubulins.Methods Cell Biol. 2010;95:77-103. doi: 10.1016/S0091-679X(10)95006-1. Methods Cell Biol. 2010. PMID: 20466131 Review.
-
Polyglutamylation: biology and analysis.Amino Acids. 2022 Apr;54(4):529-542. doi: 10.1007/s00726-022-03146-4. Epub 2022 Mar 31. Amino Acids. 2022. PMID: 35357568 Free PMC article. Review.
Cited by
-
Nna1 gene deficiency triggers Purkinje neuron death by tubulin hyperglutamylation and ER dysfunction.JCI Insight. 2020 Oct 2;5(19):e136078. doi: 10.1172/jci.insight.136078. JCI Insight. 2020. PMID: 33004692 Free PMC article.
-
Characterization of posttranslational modifications in neuron-specific class III beta-tubulin by mass spectrometry.Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4685-9. doi: 10.1073/pnas.88.11.4685. Proc Natl Acad Sci U S A. 1991. PMID: 2052551 Free PMC article.
-
Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs).J Biol Chem. 2010 Jul 23;285(30):22936-41. doi: 10.1074/jbc.C110.128280. Epub 2010 Jun 2. J Biol Chem. 2010. PMID: 20519502 Free PMC article.
-
Posttranslational modifications of alpha-tubulin of Toxoplasma gondii.Parasitol Res. 2004 Nov;94(5):386-9. doi: 10.1007/s00436-004-1220-7. Epub 2004 Oct 2. Parasitol Res. 2004. PMID: 15549389
-
Tubulin polyglutamylation is essential for airway ciliary function through the regulation of beating asymmetry.Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10490-5. doi: 10.1073/pnas.1002128107. Epub 2010 May 24. Proc Natl Acad Sci U S A. 2010. PMID: 20498047 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
