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. 2009 Aug 4;3 Suppl 6(Suppl 6):S2.
doi: 10.1186/1753-6561-3-S6-S2.

A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates

Bogdan Polevoda  1 , Thomas Arnesen, Fred Sherman
Affiliations

A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature, subunits and substrates

Bogdan Polevoda et al. BMC Proc. .

Abstract

We have introduced a consistent nomenclature for the various subunits of the NatA-NatE N-terminal acetyltransferases from yeast, humans and other eukaryotes.

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Figures

Figure 1
Figure 1
A summary of the major pathways of N-terminal processing in eukaryotes, showing the four different termini. 1: Uncleaved and unacetylated Met-Xxx- N-termini; 2: Cleaved and unacetylated Xxx-N-termini; 3: Uncleaved and NatB/NatC acetylated Ac-Met-Xxx- N-termini; 4: Cleaved and NatA acetylated Ac-Xxx-N-termini. See Table 1 and Figure 2 for more detail.
Figure 2
Figure 2
The major pathways of N-terminal processing in eukaryotes. Two methionine aminopeptidases (MAP), Map1p and Map2p, cleave N-terminal methionine residues that have small side chains (glycine, alanine, serine, cysteine, threonine, proline, and valine), although methionine is retained on some proteins having penultimate residues of valine. Subsequently, NatA, NatB, and NatC acetylate specific sequences as shown in the figure and in Table 1. Acetylation occurs at least partially on all proteins with Met-Glu-, Met-Asp- and Met-Asn- termini, but only on subclasses of proteins with the other termini. For example, acetylation occurs at least partially on 43% of proteins in yeast and on 96% of proteins in humans with Ala- termini. In addition, Ac-Cys-, Ac-Val-, Ac-Met-Met-, and Ac-Met-Lys- termini occurs on some proteins from humans but not from yeast; it is unknown which NATs are responsible for Ac-Cys-, Ac-Met-Met-, and Ac-Met-Lys- acetylations.
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