doi: 10.1016/0022-1759(91)90092-t.
A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN
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- PMID: 1960422
- DOI: 10.1016/0022-1759(91)90092-t
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A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN
J Immunol Methods.
.
Free article
Abstract
Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN.
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