Laser photoablation of guidance microchannels into hydrogels directs cell growth in three dimensions
- PMID: 19486697
- PMCID: PMC2894558
- DOI: 10.1016/j.bpj.2009.03.019
Laser photoablation of guidance microchannels into hydrogels directs cell growth in three dimensions
Abstract
Recent years have seen rapid progress in the engineering and application of biomaterials with controlled biological, physical, and chemical properties, and the development of associated methods for micropatterning of three-dimensional tissue-engineering scaffolds. A remaining challenge is the development of robust, flexible methods that can be used to create physical guidance structures in cell-seeded scaffolds independently of environmental constraints. Here we demonstrate that focal photoablation caused by pulsed lasers can generate guidance structures in transparent hydrogels, with feature control down to the micron scale. These photopatterned microchannels guide the directional growth of neurites from dorsal root ganglia. We characterize the effect of laser properties and biomaterial properties on microchannel formation in PEGylated fibrinogen hydrogels, and the effect of photoablation on neural outgrowth. This strategy could lead to the development of a new generation of guidance channels for treating nerve injuries, and the engineering of structured three-dimensional neuronal or nonneuronal networks.
Figures






Similar articles
-
The role of matrix metalloproteinases in regulating neuronal and nonneuronal cell invasion into PEGylated fibrinogen hydrogels.Biomaterials. 2010 Sep;31(25):6411-6. doi: 10.1016/j.biomaterials.2010.04.052. Biomaterials. 2010. PMID: 20537384
-
Semi-synthetic hydrogel composition and stiffness regulate neuronal morphogenesis.Int J Pharm. 2017 May 25;523(2):545-555. doi: 10.1016/j.ijpharm.2016.11.032. Int J Pharm. 2017. PMID: 28449923
-
3D bioprinting of scaffolds with living Schwann cells for potential nerve tissue engineering applications.Biofabrication. 2018 Jun 29;10(3):035014. doi: 10.1088/1758-5090/aacd30. Biofabrication. 2018. PMID: 29911990
-
Injectable, Magnetically Orienting Electrospun Fiber Conduits for Neuron Guidance.ACS Appl Mater Interfaces. 2019 Jan 9;11(1):356-372. doi: 10.1021/acsami.8b18344. Epub 2018 Dec 19. ACS Appl Mater Interfaces. 2019. PMID: 30516370 Free PMC article.
-
Compositional alterations of fibrin-based materials for regulating in vitro neural outgrowth.Tissue Eng Part A. 2008 Mar;14(3):401-11. doi: 10.1089/tea.2007.0029. Tissue Eng Part A. 2008. PMID: 18333792
Cited by
-
Hydrogels for Engineering of Perfusable Vascular Networks.Int J Mol Sci. 2015 Jul 14;16(7):15997-6016. doi: 10.3390/ijms160715997. Int J Mol Sci. 2015. PMID: 26184185 Free PMC article. Review.
-
Three-dimensional patterning of multiple cell populations through orthogonal genetic control of cell motility.Soft Matter. 2014 Apr 14;10(14):2372-80. doi: 10.1039/c3sm52265b. Soft Matter. 2014. PMID: 24622945 Free PMC article.
-
Multiphoton-Guided Creation of Complex Organ-Specific Microvasculature.Adv Healthc Mater. 2021 May;10(10):e2100031. doi: 10.1002/adhm.202100031. Epub 2021 Feb 15. Adv Healthc Mater. 2021. PMID: 33586357 Free PMC article.
-
Cytocompatible click-based hydrogels with dynamically tunable properties through orthogonal photoconjugation and photocleavage reactions.Nat Chem. 2011 Oct 23;3(12):925-31. doi: 10.1038/nchem.1174. Nat Chem. 2011. PMID: 22109271 Free PMC article.
-
Accurate flow in augmented networks (AFAN): an approach to generating three-dimensional biomimetic microfluidic networks with controlled flow.Anal Methods. 2019 Jan 7;11(1):8-16. doi: 10.1039/c8ay01798k. Epub 2018 Dec 3. Anal Methods. 2019. PMID: 31490456 Free PMC article.
References
-
- Falconnet D., Csucs G., Grandin H.M., Textor M. Surface engineering approaches to micropattern surfaces for cell-based assays. Biomaterials. 2006;27:3044–3063. - PubMed
-
- Gates B.D., Xu Q., Stewart M., Ryan D., Willson C.G. New approaches to nanofabrication: molding, printing, and other techniques. Chem. Rev. 2005;105:1171–1196. - PubMed
-
- Nie Z., Kumacheva E. Patterning surfaces with functional polymers. Nat. Mater. 2008;7:277–290. - PubMed
-
- Pedersen J.A., Swartz M.A. Mechanobiology in the third dimension. Ann. Biomed. Eng. 2005;33:1469–1490. - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources