A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA
- PMID: 1934061
- DOI: 10.1016/0092-8674(91)90531-3
A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA
Abstract
LAP, a transcriptional activator, and LIP, a transcriptional repressor, are translated from a single mRNA species by using two AUGs within the same reading frame. These two proteins share the 145 C-terminal amino acids that contain the basic DNA-binding domain and the leucine zipper dimerization helix. Probably owing to its higher affinity for its DNA cognate sequences, LIP can attenuate the transcriptional stimulation by LAP in substoichiometric amounts. As revealed by transient transfection experiments, a moderate increase in the LAP/LIP ratio results in a significantly higher transcriptional activation of an appropriate target gene. The LAP/LIP ratio increases about 5-fold during terminal rat liver differentiation and is thus likely to modulate the activity of LAP in the intact animal.
Similar articles
-
CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8219-23. doi: 10.1073/pnas.90.17.8219. Proc Natl Acad Sci U S A. 1993. PMID: 8367486 Free PMC article.
-
P53 regulates CCAAT/Enhancer binding protein β gene expression.Gene. 2023 Oct 30;884:147675. doi: 10.1016/j.gene.2023.147675. Epub 2023 Aug 2. Gene. 2023. PMID: 37541559
-
CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription.Genes Dev. 1992 Mar;6(3):439-53. doi: 10.1101/gad.6.3.439. Genes Dev. 1992. PMID: 1547942
-
Transcriptional regulation of the human hepatic CYP3A4: identification of a new distal enhancer region responsive to CCAAT/enhancer-binding protein beta isoforms (liver activating protein and liver inhibitory protein).Mol Pharmacol. 2005 Jun;67(6):2088-101. doi: 10.1124/mol.104.008169. Epub 2005 Mar 18. Mol Pharmacol. 2005. PMID: 15778453
-
Regulation of liver-specific gene expression.Cell Struct Funct. 1993 Aug;18(4):189-94. doi: 10.1247/csf.18.189. Cell Struct Funct. 1993. PMID: 8293495 Review. No abstract available.
Cited by
-
Critical role for CCAAT/enhancer-binding protein β in immune complex-induced acute lung injury.J Immunol. 2012 Aug 1;189(3):1480-90. doi: 10.4049/jimmunol.1200877. Epub 2012 Jun 25. J Immunol. 2012. PMID: 22732594 Free PMC article.
-
Regulation of CCAAT/enhancer-binding protein-beta isoform synthesis by alternative translational initiation at multiple AUG start sites.Nucleic Acids Res. 2001 Jul 15;29(14):3087-98. doi: 10.1093/nar/29.14.3087. Nucleic Acids Res. 2001. PMID: 11452034 Free PMC article.
-
Role of very-late antigen-4 (VLA-4) in myelin basic protein-primed T cell contact-induced expression of proinflammatory cytokines in microglial cells.J Biol Chem. 2003 Jun 20;278(25):22424-31. doi: 10.1074/jbc.M301789200. Epub 2003 Apr 10. J Biol Chem. 2003. PMID: 12690109 Free PMC article.
-
Adipogenic signaling in rat white adipose tissue: modulation by aging and calorie restriction.Exp Gerontol. 2007 Aug;42(8):733-44. doi: 10.1016/j.exger.2007.05.011. Epub 2007 Jun 6. Exp Gerontol. 2007. PMID: 17624709 Free PMC article.
-
S-nitrosoglutathione inhibits adipogenesis in 3T3-L1 preadipocytes by S-nitrosation of CCAAT/enhancer-binding protein β.Sci Rep. 2019 Oct 28;9(1):15403. doi: 10.1038/s41598-019-51579-x. Sci Rep. 2019. PMID: 31659183 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
