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. 2009 May;91(5):620-3.
doi: 10.1016/j.biochi.2009.03.005. Epub 2009 Mar 21.

Transmembrane cholesterol migration in planar lipid membranes measured with Vibrio cholerae cytolysin as molecular tool

Oleg V Krasilnikov  1 , Liliya N Yuldasheva
Affiliations

Transmembrane cholesterol migration in planar lipid membranes measured with Vibrio cholerae cytolysin as molecular tool

Oleg V Krasilnikov et al. Biochimie. 2009 May.

Abstract

The rate of transbilayer movement (flip-flop) of cholesterol was estimated using planar bilayers with defined initial asymmetry, formed by the opposing monolayers technique. Vibrio cholerae cytolysin (VCC) was utilized as a molecular tool for measuring the cholesterol concentration in the cis leaflet of asymmetric bilayers. To quantify cholesterol flip-flop in planar lipid bilayers, a mathematical model was developed. It considers both the lateral diffusion rate of cholesterol within each monolayer and the flip-flop rate. The difference in initial and steady-state cholesterol contents in bilayer leaflets was used as a start point. Assuming the lateral diffusion coefficient to be of 1 x 10(-8) cm(2) s(-1), the characteristic time of cholesterol flip-flop at 25 +/- 2 degrees C was estimated as <10 s.

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