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. 1977 Apr;22(1):1-8.
doi: 10.1128/JVI.22.1.1-8.1977.

Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein

J Luka, W Siegert, G Klein

Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein

J Luka et al. J Virol. 1977 Apr.

Abstract

The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.

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