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. 2009 Mar;15(3):469-71.
doi: 10.3201/eid1503.081104.

Coordinated implementation of chikungunya virus reverse transcription-PCR

Marcus Panning  1 , Remi N Charrel, Oliver Donoso Mantke, Olfert Landt, Matthias Niedrig, Christian Drosten
Affiliations

Coordinated implementation of chikungunya virus reverse transcription-PCR

Marcus Panning et al. Emerg Infect Dis. 2009 Mar.

Erratum in

  • Emerg Infect Dis. 2009 Aug;15(8):1334. Mantke, Oliver D [corrected to Donoso Mantke, Oliver]

Abstract

A preformulated chikungunya virus real-time reverse transcription-PCR, quality-confirmed oligonucleotides, and noninfectious virus controls were distributed by the European Network for the Diagnosis of Imported Viral Diseases. An international proficiency study with 31 participants demonstrated that ad hoc implementation of molecular diagnostics was feasible and successful.

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Figures

Figure
Figure
Probit analysis of laboratories with a positive result (y axes) for chikungunya virus in relation to viral RNA concentration in positive samples (x axes). A) Laboratories using in-house reverse transcription–PCRs (RT-PCRs) (n = 18) had a 50% certainty of having a positive result at 10,000 RNA copies/mL (95% confidence interval [CI] 3,162–19,952). B) Laboratories using a preformulated RT-PCR (n = 13) had a 50% certainty of having a positive result at 1,288 RNA copies/mL (95% CI 416–2,344). Data points represent individual samples in the test panel. Thick line is the regression line calculated on the basis of a probit model (dose-response curve), and thin lines are 95% CIs. Data fit into the model with p<0.00001.
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