Warfarin administration disrupts the assembly of mineralized nodules in the osteoid
- PMID: 19225034
- DOI: 10.1093/jmicro/dfp008
Warfarin administration disrupts the assembly of mineralized nodules in the osteoid
Abstract
This study aimed to elucidate the ultrastructural role of Gla proteins in bone mineralization by means of a warfarin-administration model. Thirty-six 4-week-old male F344 rats received warfarin (warfarin group) or distilled water (control group), and were fixed after 4, 8 and 12 weeks with an aldehyde solution. Tibiae and femora were employed for histochemical analyses of alkaline phosphatase, osteocalcin and tartrate-resistant acid phosphatase, and for bone histomorphometry and electron microscopy. After 4, 8 and 12 weeks, there were no marked histochemical and histomorphometrical differences between control and warfarin groups. However, osteocalcin immunoreactivity was markedly reduced in the warfarin-administered bone. Mineralized nodules and globular assembly of crystalline particles were seen in the control osteoid. Alternatively, warfarin administration resulted in crystalline particles being dispersed throughout the osteoid without forming mineralized nodules. Immunoelectron microscopy unveiled lower osteocalcin content in the warfarin-administered osteoid, which featured scattered crystalline particles, whereas osteocalcin was abundant on the normally mineralized nodules in the control osteoid. In summary, Gla proteins appear to play a pivotal role in the assembly of mineralized nodules.
Similar articles
-
Vitamin K2, a gamma-carboxylating factor of gla-proteins, normalizes the bone crystal nucleation impaired by Mg-insufficiency.Histol Histopathol. 2008 Nov;23(11):1353-66. doi: 10.14670/HH-23.1353. Histol Histopathol. 2008. PMID: 18785118
-
A histomorphometric, structural, and immunocytochemical study of the effects of diet-induced hypocalcemia on bone in growing rats.J Histochem Cytochem. 2000 Aug;48(8):1059-78. doi: 10.1177/002215540004800804. J Histochem Cytochem. 2000. PMID: 10898800
-
[Biological effects of vitamin K2 on bone quality].Clin Calcium. 2009 Dec;19(12):1788-96. Clin Calcium. 2009. PMID: 19949270 Review. Japanese.
-
Altered distribution of bone matrix proteins and defective bone mineralization in klotho-deficient mice.Bone. 2013 Nov;57(1):206-19. doi: 10.1016/j.bone.2013.08.008. Epub 2013 Aug 14. Bone. 2013. PMID: 23954506
-
Developmental appearance of Gla proteins (osteocalcin) and alkaline phosphatase in tooth germs and bones of the rat.Bone Miner. 1987 Aug;2(5):361-73. Bone Miner. 1987. PMID: 3333320
Cited by
-
Guidelines for the use of bone metabolic markers in the diagnosis and treatment of osteoporosis (2012 edition).J Bone Miner Metab. 2013 Jan;31(1):1-15. doi: 10.1007/s00774-012-0392-y. Epub 2012 Nov 10. J Bone Miner Metab. 2013. PMID: 23143508 Review.
-
Joint Degradation in a Monkey Model of Collagen-Induced Arthritis: Role of Cathepsin K Based on Biochemical Markers and Histological Evaluation.Int J Rheumatol. 2016;2016:8938916. doi: 10.1155/2016/8938916. Epub 2016 Feb 2. Int J Rheumatol. 2016. PMID: 26949397 Free PMC article.
-
Vitamin K-Dependent Carboxylation of Osteocalcin in Bone-Ally or Adversary of Bone Mineral Status in Rats with Experimental Chronic Kidney Disease?Nutrients. 2022 Oct 1;14(19):4082. doi: 10.3390/nu14194082. Nutrients. 2022. PMID: 36235734 Free PMC article.
-
Ultrastructure and biological function of matrix vesicles in bone mineralization.Histochem Cell Biol. 2018 Apr;149(4):289-304. doi: 10.1007/s00418-018-1646-0. Epub 2018 Feb 6. Histochem Cell Biol. 2018. PMID: 29411103 Review.
-
Ultrastructural and biochemical aspects of matrix vesicle-mediated mineralization.Jpn Dent Sci Rev. 2017 May;53(2):34-45. doi: 10.1016/j.jdsr.2016.09.002. Epub 2016 Nov 5. Jpn Dent Sci Rev. 2017. PMID: 28479934 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
