doi: 10.1016/S0076-6879(08)03604-5.
Assaying of autophagic protein degradation
Affiliations
- PMID: 19200875
- DOI: 10.1016/S0076-6879(08)03604-5
Item in Clipboard
Assaying of autophagic protein degradation
Methods Enzymol.
2009.
Abstract
Macroautophagy is a three-step process: (1) autophagosomes form and mature, (2) the autophagosomes fuse with lysosomes, and (3) the autophagic cargo is degraded in the lysosomes. It is this lysosomal degradation of the autophagic cargo that constitutes the autophagic flux. As in the case of metabolic pathways, the steady-state concentration of the intermediary autophagic structures alone is insufficient for investigating the flux. Assaying the degradation of long-lived proteins as described in this chapter is one of the methods that can be used to measure autophagic flux.
Similar articles
-
Sequestration assays for mammalian autophagy.Methods Enzymol. 2009;452:63-83. doi: 10.1016/S0076-6879(08)03605-7. Methods Enzymol. 2009. PMID: 19200876
-
Autophagic proteolysis of long-lived proteins in nonliver cells.Methods Mol Biol. 2008;445:111-7. doi: 10.1007/978-1-59745-157-4_6. Methods Mol Biol. 2008. PMID: 18425445
-
Ultrastructural and immunocytochemical characterization of autophagic vacuoles in isolated hepatocytes: effects of vinblastine and asparagine on vacuole distributions.Exp Cell Res. 1995 Dec;221(2):504-19. doi: 10.1006/excr.1995.1402. Exp Cell Res. 1995. PMID: 7493651
-
Methods for monitoring autophagy.Int J Biochem Cell Biol. 2004 Dec;36(12):2491-502. doi: 10.1016/j.biocel.2004.02.005. Int J Biochem Cell Biol. 2004. PMID: 15325587 Review.
-
Aging: central role for autophagy and the lysosomal degradative system.Ageing Res Rev. 2009 Jul;8(3):199-213. doi: 10.1016/j.arr.2009.05.001. Epub 2009 May 7. Ageing Res Rev. 2009. PMID: 19427410 Review.
Cited by
-
The insufficiency of ATG4A in macroautophagy.J Biol Chem. 2020 Sep 25;295(39):13584-13600. doi: 10.1074/jbc.RA120.013897. Epub 2020 Jul 30. J Biol Chem. 2020. PMID: 32732290 Free PMC article.
-
Why should autophagic flux be assessed?Acta Pharmacol Sin. 2013 May;34(5):595-9. doi: 10.1038/aps.2012.184. Epub 2013 Mar 11. Acta Pharmacol Sin. 2013. PMID: 23474710 Free PMC article. Review.
-
Autophagy: assays and artifacts.J Pathol. 2010 Jun;221(2):117-24. doi: 10.1002/path.2694. J Pathol. 2010. PMID: 20225337 Free PMC article. Review.
-
Autophagy Is Involved in Mesenchymal Stem Cell Death in Coculture with Chondrocytes.Cartilage. 2021 Dec;13(2_suppl):969S-979S. doi: 10.1177/1947603520941227. Epub 2020 Jul 22. Cartilage. 2021. PMID: 32693629 Free PMC article.
-
Identification of modulators of autophagic flux in an image-based high content siRNA screen.Autophagy. 2016;12(4):713-26. doi: 10.1080/15548627.2016.1147669. Autophagy. 2016. PMID: 27050463 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
