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. 2008 Dec;40(12):1413-5.
doi: 10.1038/ng.259. Epub 2008 Nov 2.

Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing

Qun Pan  1 , Ofer Shai, Leo J Lee, Brendan J Frey, Benjamin J Blencowe
Affiliations

Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing

Qun Pan et al. Nat Genet. 2008 Dec.

Erratum in

  • Nat Genet. 2009 Jun;41(6):762

Abstract

We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in approximately 20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from approximately 95% of multiexon genes undergo alternative splicing and that there are approximately 100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.

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