Solution NMR structure determination of proteins revisited

doi:10.1007/s10858-008-9277-8

Review

. 2008 Nov;42(3):155-8.

doi: 10.1007/s10858-008-9277-8. Epub 2008 Oct 1.

Solution NMR structure determination of proteins revisited

Martin Billeter¹, Gerhard Wagner, Kurt Wüthrich

Affiliations

PMID: 18827972
PMCID: PMC2748914
DOI: 10.1007/s10858-008-9277-8

Review

Solution NMR structure determination of proteins revisited

Martin Billeter et al. J Biomol NMR. 2008 Nov.

. 2008 Nov;42(3):155-8.

doi: 10.1007/s10858-008-9277-8. Epub 2008 Oct 1.

Authors

Martin Billeter¹, Gerhard Wagner, Kurt Wüthrich

Affiliation

¹ Department of Chemistry, University of Gothenburg, Box 462, 40530, Gothenburg, Sweden. martin.billeter@chem.gu.se

PMID: 18827972
PMCID: PMC2748914
DOI: 10.1007/s10858-008-9277-8

Abstract

This 'Perspective' bears on the present state of protein structure determination by NMR in solution. The focus is on a comparison of the infrastructure available for NMR structure determination when compared to protein crystal structure determination by X-ray diffraction. The main conclusion emerges that the unique potential of NMR to generate high resolution data also on dynamics, interactions and conformational equilibria has contributed to a lack of standard procedures for structure determination which would be readily amenable to improved efficiency by automation. To spark renewed discussion on the topic of NMR structure determination of proteins, procedural steps with high potential for improvement are identified.

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References

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[1] Acton TB, Gunsalus KC, Xiao R, Ma LC, Aramini J, Baran MC, Chiang YW, Climent T, Cooper B, Denissova NG, Douglas SM, Everett JK, Ho CK, Macapagal D, Rajan PK, Shastry R, Shih LY, Swapna GV, Wilson M, Wu M, Gerstein M, Inouye M, Hunt JF, Montelione GT. Robotic cloning and protein production platform of the Northeast Structural Genomics Consortium. Meth Enzymol. 2005;394:210–243. - PubMed

[2] Acton TB, Gunsalus KC, Xiao R, Ma LC, Aramini J, Baran MC, Chiang YW, Climent T, Cooper B, Denissova NG, Douglas SM, Everett JK, Ho CK, Macapagal D, Rajan PK, Shastry R, Shih LY, Swapna GV, Wilson M, Wu M, Gerstein M, Inouye M, Hunt JF, Montelione GT. Robotic cloning and protein production platform of the Northeast Structural Genomics Consortium. Meth Enzymol. 2005;394:210–243. - PubMed

[3] Bagby S, Tong KI, Ikura M. Optimization of protein solubility and stability for protein nuclear magnetic resonance. Methods Enzymol. 2001;339:20–41. - PubMed

[4] Bagby S, Tong KI, Ikura M. Optimization of protein solubility and stability for protein nuclear magnetic resonance. Methods Enzymol. 2001;339:20–41. - PubMed

[5] Bagby S, Tong KI, Liu D, Alattia JR, Ikura M. The button test: a small scale method using microdialysis cells for assessing protein solubility at concentrations suitable for NMR. J Biomol NMR. 1997;10:279–282. - PubMed

[6] Bagby S, Tong KI, Liu D, Alattia JR, Ikura M. The button test: a small scale method using microdialysis cells for assessing protein solubility at concentrations suitable for NMR. J Biomol NMR. 1997;10:279–282. - PubMed

[7] Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN. The Protein Data Bank. Nucl Acids Res. 2000;28:235–242. - PMC - PubMed

[8] Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN. The Protein Data Bank. Nucl Acids Res. 2000;28:235–242. - PMC - PubMed

[9] Bhattacharya A, Tejero R, Montelione G. Evaluating protein structures determined by structural genomics consortia. Proteins. 2007;66:778–795. - PubMed

[10] Bhattacharya A, Tejero R, Montelione G. Evaluating protein structures determined by structural genomics consortia. Proteins. 2007;66:778–795. - PubMed

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Solution NMR structure determination of proteins revisited

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Solution NMR structure determination of proteins revisited

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