Assembling the human IFN-beta enhanceosome in solution
- PMID: 18823997
- DOI: 10.1016/j.jmb.2008.09.015
Assembling the human IFN-beta enhanceosome in solution
Abstract
Assembly of interferon-beta enhanceosome from its individual protein components and of enhancer DNA has been studied in solution using a combination of fluorescence anisotropy, microcalorimetry, and CD titration. It was shown that the enhancer binds only one full-length phosphomimetic IRF-3 dimer at the PRDIII-PRDI sites, and this binding does not exhibit cooperativity with binding of the ATF-2/c-Jun bZIP (leucine zipper dimer with basic DNA recognition segments) heterodimer at the PRDIV site. The orientation of the bZIP pair is, therefore, not determined by the presence of the IRF-3 dimer, but is predetermined by the asymmetry of the PRDIV site. In contrast, bound IRF-3 dimer interacts strongly with the NF-kappaB (p50/p65) heterodimer bound at the neighboring PRDII site. The orientation of bound NF-kappaB is also predetermined by the asymmetry of the PRDII site and is the opposite of that found in the crystal structure. The HMG-I/Y protein, proposed as orchestrating enhanceosome assembly, interacts specifically with the PRDII site of the interferon-beta enhancer by inserting its DNA-binding segments (AT hooks) into the minor groove, resulting in a significant increase in NF-kappaB binding affinity for the major groove of this site.
Similar articles
-
Assembly of a functional beta interferon enhanceosome is dependent on ATF-2-c-jun heterodimer orientation.Mol Cell Biol. 2000 Jul;20(13):4814-25. doi: 10.1128/MCB.20.13.4814-4825.2000. Mol Cell Biol. 2000. PMID: 10848607 Free PMC article.
-
Stability and DNA-binding ability of the bZIP dimers formed by the ATF-2 and c-Jun transcription factors.J Mol Biol. 2010 Feb 19;396(2):431-40. doi: 10.1016/j.jmb.2009.11.050. Epub 2009 Nov 26. J Mol Biol. 2010. PMID: 19944700
-
Crystal structure of ATF-2/c-Jun and IRF-3 bound to the interferon-beta enhancer.EMBO J. 2004 Nov 10;23(22):4384-93. doi: 10.1038/sj.emboj.7600453. Epub 2004 Oct 28. EMBO J. 2004. PMID: 15510218 Free PMC article.
-
The enhanceosome.Curr Opin Struct Biol. 2008 Apr;18(2):236-42. doi: 10.1016/j.sbi.2007.12.002. Epub 2008 Feb 21. Curr Opin Struct Biol. 2008. PMID: 18206362 Review.
-
Molecular mimicry of the NF-kappaB DNA target site by a selected RNA aptamer.Curr Opin Struct Biol. 2004 Feb;14(1):21-7. doi: 10.1016/j.sbi.2004.01.004. Curr Opin Struct Biol. 2004. PMID: 15102445 Review.
Cited by
-
Structural insights into interferon regulatory factor activation.Cell Signal. 2010 Jun;22(6):883-7. doi: 10.1016/j.cellsig.2009.12.005. Epub 2009 Dec 30. Cell Signal. 2010. PMID: 20043992 Free PMC article. Review.
-
Genome reading by the NF-κB transcription factors.Nucleic Acids Res. 2019 Nov 4;47(19):9967-9989. doi: 10.1093/nar/gkz739. Nucleic Acids Res. 2019. PMID: 31501881 Free PMC article. Review.
-
Characterization of PicoGreen interaction with dsDNA and the origin of its fluorescence enhancement upon binding.Biophys J. 2010 Nov 3;99(9):3010-9. doi: 10.1016/j.bpj.2010.09.012. Biophys J. 2010. PMID: 21044599 Free PMC article.
-
Thermodynamics of cooperative DNA recognition at a replication origin and transcription regulatory site.Biochemistry. 2010 Dec 7;49(48):10277-86. doi: 10.1021/bi1014908. Epub 2010 Nov 10. Biochemistry. 2010. PMID: 21047141 Free PMC article.
-
Arabidopsis thaliana AHL family modulates hypocotyl growth redundantly by interacting with each other via the PPC/DUF296 domain.Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):E4688-97. doi: 10.1073/pnas.1219277110. Epub 2013 Nov 11. Proc Natl Acad Sci U S A. 2013. PMID: 24218605 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
