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. 1976 Nov;73(11):3872-6.
doi: 10.1073/pnas.73.11.3872.

DNA gyrase: an enzyme that introduces superhelical turns into DNA

M Gellert, K Mizuuchi, M H O'Dea, H A Nash

DNA gyrase: an enzyme that introduces superhelical turns into DNA

M Gellert et al. Proc Natl Acad Sci U S A. 1976 Nov.

Abstract

Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.

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