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. 2008 Sep 4;455(7209):58-63.
doi: 10.1038/nature07228. Epub 2008 Jul 30.

Widespread changes in protein synthesis induced by microRNAs

Matthias Selbach  1 , Björn Schwanhäusser, Nadine Thierfelder, Zhuo Fang, Raya Khanin, Nikolaus Rajewsky
Affiliations

Widespread changes in protein synthesis induced by microRNAs

Matthias Selbach et al. Nature. .

Abstract

Animal microRNAs (miRNAs) regulate gene expression by inhibiting translation and/or by inducing degradation of target messenger RNAs. It is unknown how much translational control is exerted by miRNAs on a genome-wide scale. We used a new proteomic approach to measure changes in synthesis of several thousand proteins in response to miRNA transfection or endogenous miRNA knockdown. In parallel, we quantified mRNA levels using microarrays. Here we show that a single miRNA can repress the production of hundreds of proteins, but that this repression is typically relatively mild. A number of known features of the miRNA-binding site such as the seed sequence also govern repression of human protein synthesis, and we report additional target sequence characteristics. We demonstrate that, in addition to downregulating mRNA levels, miRNAs also directly repress translation of hundreds of genes. Finally, our data suggest that a miRNA can, by direct or indirect effects, tune protein synthesis from thousands of genes.

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