Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008:(39):25-31.
doi: 10.1093/jncimonographs/lgn011.

Characterization of MYC translocations in multiple myeloma cell lines

Amel Dib  1 , Ana Gabrea, Oleg K Glebov, P Leif Bergsagel, W Michael Kuehl
Affiliations

Characterization of MYC translocations in multiple myeloma cell lines

Amel Dib et al. J Natl Cancer Inst Monogr. 2008.

Abstract

Translocations involving an MYC gene (c >> N >>L) are very late tumor progression events and provide a paradigm for secondary translocations in multiple myeloma. Using a combination of fluorescent in situ hybridization and comparative genomic hybridization arrays (aCGH), we have identified rearrangements of an MYC gene in 40 of 43 independent myeloma cell lines. A majority of MYC translocations involve an Ig locus (IgH > Iglambda >> Igkappa), but the breakpoints only infrequently occur near or within switch regions or V(D)J sequences. Surprisingly, about 40% of MYC translocations do not involve an Ig locus. The MYC translocations mostly are nonreciprocal translocations or insertions, often with the involvement of three chromosomes and sometimes with associated duplication, amplification, inversion, and other associated chromosomal abnormalities. High-density aCGH analyses should facilitate the cloning of MYC breakpoints, enabling the determination of their structures and perhaps elucidating how rearrangements not involving an Ig gene cause dysregulation of an MYC gene.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Examples of molecular cytogenetic analyses of MYC rearrangements in multiple myeloma. A, B) Primary data and C) schematic representations of FISH analyses of metaphase chromosomes found in different cell lines. MYC probe is red and Ig C (3′ enhancer) probe is green. A) Normal chromosome 8 (CHR 8) and amplification of MYC gene and flanking sequences on der(8) in KAS-6 HMCL; B) normal chromosome 2 (CHR 2) and juxtaposition of Ck probe from 2p12 near N-MYC at 2p25 on inv(2)(p25p12) in PE-2 HMCL. The Cκ probe cross-hybridizes with unknown sequences at 2q11 on both CHR 2 and on inv(2)(p25p12). C) Diagrams of some of the structural rearrangements involving c-MYC that have been identified by FISH analyses in different HMCL and primary MM tumor samples.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bergsagel PL, Kuehl WM. Molecular pathogenesis and a consequent classification of multiple myeloma. J Clin Oncol. 2005;23(26):6333–6338. - PubMed
    1. Bergsagel PL, Kuehl WM, Zhan F, Sawyer J, Barlogie B, Shaughnessy J., Jr Cyclin D dysregulation: an early and unifying pathogenic event in multiple myeloma. Blood. 2005;106(1):296–303. - PMC - PubMed
    1. Fonseca R, Bailey RJ, Ahmann GJ, et al. Genomic abnormalities in monoclonal gammopathy of undetermined significance. Blood. 2002;100(4):1417–1424. - PubMed
    1. Fonseca R, Barlogie B, Bataille R, et al. Genetics and cytogenetics of multiple myeloma: a workshop report. Cancer Res. 2004;64(4):1546–1558. - PubMed
    1. Bergsagel PL, Kuehl WM. Chromosomal translocations in multiple myeloma. Oncogene. 2001;20(40):5611–5622. - PubMed

MeSH terms

Substances