Alpha-actinin-4 is selectively required for insulin-induced GLUT4 translocation
- PMID: 18617516
- DOI: 10.1074/jbc.M801750200
Alpha-actinin-4 is selectively required for insulin-induced GLUT4 translocation
Abstract
Insulin induces GLUT4 translocation to the muscle cell surface. Using differential amino acid labeling and mass spectrometry, we observed insulin-dependent co-precipitation of actinin-4 (ACTN4) with GLUT4 (Foster, L. J., Rudich, A., Talior, I., Patel, N., Huang, X., Furtado, L. M., Bilan, P. J., Mann, M., and Klip, A. (2006) J. Proteome Res. 5, 64-75). ACTN4 links F-actin to membrane proteins, and actin dynamics are essential for GLUT4 translocation. We hypothesized that ACTN4 may contribute to insulin-regulated GLUT4 traffic. In L6 muscle cells insulin, but not platelet-derived growth factor, increased co-precipitation of ACTN4 with GLUT4. Small interfering RNA-mediated ACTN4 knockdown abolished the gain in surface-exposed GLUT4 elicited by insulin but not by platelet-derived growth factor, membrane depolarization, or mitochondrial uncoupling. In contrast, knockdown of alpha-actinin-1 (ACTN1) did not prevent GLUT4 translocation by insulin. GLUT4 colocalized with ACTN4 along the insulin-induced cortical actin mesh and ACTN4 knockdown prevented GLUT4-actin colocalization without impeding actin remodeling or Akt phosphorylation, maintaining GLUT4 in a tight perinuclear location. We propose that ACTN4 contributes to GLUT4 traffic, likely by tethering GLUT4 vesicles to the cortical actin cytoskeleton.
Similar articles
-
A complex of Rab13 with MICAL-L2 and α-actinin-4 is essential for insulin-dependent GLUT4 exocytosis.Mol Biol Cell. 2016 Jan 1;27(1):75-89. doi: 10.1091/mbc.E15-05-0319. Epub 2015 Nov 4. Mol Biol Cell. 2016. PMID: 26538022 Free PMC article.
-
Insulin but not PDGF relies on actin remodeling and on VAMP2 for GLUT4 translocation in myoblasts.J Cell Sci. 2004 Oct 15;117(Pt 22):5447-55. doi: 10.1242/jcs.01421. Epub 2004 Oct 5. J Cell Sci. 2004. PMID: 15466888
-
Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC).J Proteome Res. 2006 Jan;5(1):64-75. doi: 10.1021/pr0502626. J Proteome Res. 2006. PMID: 16396496
-
Signal transduction meets vesicle traffic: the software and hardware of GLUT4 translocation.Am J Physiol Cell Physiol. 2014 May 15;306(10):C879-86. doi: 10.1152/ajpcell.00069.2014. Epub 2014 Mar 5. Am J Physiol Cell Physiol. 2014. PMID: 24598362 Review.
-
Update on GLUT4 Vesicle Traffic: A Cornerstone of Insulin Action.Trends Endocrinol Metab. 2017 Aug;28(8):597-611. doi: 10.1016/j.tem.2017.05.002. Epub 2017 Jun 8. Trends Endocrinol Metab. 2017. PMID: 28602209 Review.
Cited by
-
Combinatorial morphogenesis of dendritic spines and filopodia by SPAR and alpha-actinin2.Biochem Biophys Res Commun. 2009 Jun 19;384(1):55-60. doi: 10.1016/j.bbrc.2009.04.069. Epub 2009 Apr 23. Biochem Biophys Res Commun. 2009. PMID: 19393616 Free PMC article.
-
Mitochondrial dysfunction and insulin resistance from the outside in: extracellular matrix, the cytoskeleton, and mitochondria.Am J Physiol Endocrinol Metab. 2011 Nov;301(5):E749-55. doi: 10.1152/ajpendo.00363.2011. Epub 2011 Aug 23. Am J Physiol Endocrinol Metab. 2011. PMID: 21862724 Free PMC article. Review.
-
Alström syndrome: current perspectives.Appl Clin Genet. 2015 Jul 21;8:171-9. doi: 10.2147/TACG.S56612. eCollection 2015. Appl Clin Genet. 2015. PMID: 26229500 Free PMC article. Review.
-
Signaling, cytoskeletal and membrane mechanisms regulating GLUT4 exocytosis.Trends Endocrinol Metab. 2011 Mar;22(3):110-6. doi: 10.1016/j.tem.2010.12.001. Epub 2011 Jan 7. Trends Endocrinol Metab. 2011. PMID: 21216617 Free PMC article. Review.
-
Myo1c binding to submembrane actin mediates insulin-induced tethering of GLUT4 vesicles.Mol Biol Cell. 2012 Oct;23(20):4065-78. doi: 10.1091/mbc.E12-04-0263. Epub 2012 Aug 23. Mol Biol Cell. 2012. PMID: 22918957 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
