Fibronectin and VLA-4 in haematopoietic stem cell-microenvironment interactions
- PMID: 1861722
- DOI: 10.1038/352438a0
Fibronectin and VLA-4 in haematopoietic stem cell-microenvironment interactions
Abstract
The self-renewal and differentiation of haematopoietic stem cells occurs in vivo and in vitro in direct contact with cells making up the haematopoietic microenvironment. In this study we used adhesive ligands and blocking antibodies to identify stromal cell-derived extracellular matrix proteins involved in promoting attachment of murine haematopoietic stem cells. Here we report that day-12 colony-forming-unit spleen (CFU-S12)5 cells and reconstituting haematopoietic stem cells attach to the C-terminal, heparin-binding fragment of fibronectin by recognizing the CS-1 peptide of the alternatively spliced non-type III connecting segment (IIICS) of human plasma fibronectin. Furthermore, CFU-S12 stem cells express the alpha 4 subunit of the VLA-4 integrin receptor, which is known to be a receptor for the CS-1 sequence, and monoclonal antibodies against the integrin alpha 4 subunit of VLA-4 block adhesion of CFU-S12 stem cells to plates coated with the C-terminal fibronectin fragment. Finally, polyclonal antibodies against the integrin beta 1 subunit of VLA-4 inhibit the formation of CFU-S12-derived spleen colonies and medullary haematopoiesis in vivo following intravenous infusion of antibody-treated bone marrow cells.
Similar articles
-
Fibronectin increases both non-adherent cells and CFU-GM while collagen increases adherent cells in human normal long-term bone marrow cultures.Haematologia (Budap). 1997;28(2):77-84. Haematologia (Budap). 1997. PMID: 9283907
-
Hepatocyte growth factor/scatter factor (HGF/SF) is produced by human bone marrow stromal cells and promotes proliferation, adhesion and survival of human hematopoietic progenitor cells (CD34+).Exp Hematol. 1998 Aug;26(9):885-94. Exp Hematol. 1998. PMID: 9694510
-
Costimulation of proliferative responses of resting CD4+ T cells by the interaction of VLA-4 and VLA-5 with fibronectin or VLA-6 with laminin.J Immunol. 1990 Jul 1;145(1):59-67. J Immunol. 1990. PMID: 1972721
-
Cell-cell matrix and cell-cell interactions during hematopoietic differentiation.Bone Marrow Transplant. 1992;9 Suppl 1:19-22. Bone Marrow Transplant. 1992. PMID: 1504666 Review. No abstract available.
-
Novel cell surface adhesion receptors involved in interactions between stromal macrophages and haematopoietic cells.J Cell Sci Suppl. 1988;9:185-206. doi: 10.1242/jcs.1988.supplement_9.10. J Cell Sci Suppl. 1988. PMID: 3077137 Review.
Cited by
-
Rac signaling in osteoblastic cells is required for normal bone development but is dispensable for hematopoietic development.Blood. 2012 Jan 19;119(3):736-44. doi: 10.1182/blood-2011-07-368753. Epub 2011 Nov 28. Blood. 2012. PMID: 22123845 Free PMC article.
-
Homing in hematopoietic stem cells: focus on regulatory role of CXCR7 on SDF1a/CXCR4 axis.EXCLI J. 2016 Feb 15;15:134-43. doi: 10.17179/excli2014-585. eCollection 2016. EXCLI J. 2016. PMID: 27092040 Free PMC article. Review.
-
Progress and prospects for stem cell engineering.Annu Rev Chem Biomol Eng. 2011;2:479-502. doi: 10.1146/annurev-chembioeng-061010-114105. Annu Rev Chem Biomol Eng. 2011. PMID: 22432628 Free PMC article. Review.
-
Bcr-Abl is a "molecular switch" for the decision for growth and differentiation in hematopoietic stem cells.Int J Hematol. 2002 Jul;76(1):35-43. doi: 10.1007/BF02982716. Int J Hematol. 2002. PMID: 12138893 Review.
-
Bcr-Abl induces abnormal cytoskeleton remodeling, beta1 integrin clustering and increased cell adhesion to fibronectin through the Abl interactor 1 pathway.J Cell Sci. 2007 Apr 15;120(Pt 8):1436-46. doi: 10.1242/jcs.03430. Epub 2007 Mar 27. J Cell Sci. 2007. PMID: 17389688 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous
