Next-generation DNA sequencing methods
- PMID: 18576944
- DOI: 10.1146/annurev.genom.9.081307.164359
Next-generation DNA sequencing methods
Abstract
Recent scientific discoveries that resulted from the application of next-generation DNA sequencing technologies highlight the striking impact of these massively parallel platforms on genetics. These new methods have expanded previously focused readouts from a variety of DNA preparation protocols to a genome-wide scale and have fine-tuned their resolution to single base precision. The sequencing of RNA also has transitioned and now includes full-length cDNA analyses, serial analysis of gene expression (SAGE)-based methods, and noncoding RNA discovery. Next-generation sequencing has also enabled novel applications such as the sequencing of ancient DNA samples, and has substantially widened the scope of metagenomic analysis of environmentally derived samples. Taken together, an astounding potential exists for these technologies to bring enormous change in genetic and biological research and to enhance our fundamental biological knowledge.
Similar articles
-
Applications of ultra-high-throughput sequencing.Methods Mol Biol. 2009;553:79-108. doi: 10.1007/978-1-60327-563-7_5. Methods Mol Biol. 2009. PMID: 19588102 Review.
-
Applications of next-generation sequencing technologies in functional genomics.Genomics. 2008 Nov;92(5):255-64. doi: 10.1016/j.ygeno.2008.07.001. Epub 2008 Aug 24. Genomics. 2008. PMID: 18703132 Review.
-
Next-generation sequencing and potential applications in fungal genomics.Methods Mol Biol. 2011;722:51-60. doi: 10.1007/978-1-61779-040-9_4. Methods Mol Biol. 2011. PMID: 21590412
-
Applications of new sequencing technologies for transcriptome analysis.Annu Rev Genomics Hum Genet. 2009;10:135-51. doi: 10.1146/annurev-genom-082908-145957. Annu Rev Genomics Hum Genet. 2009. PMID: 19715439 Review.
-
What can next generation sequencing do for you? Next generation sequencing as a valuable tool in plant research.Plant Biol (Stuttg). 2010 Nov;12(6):831-41. doi: 10.1111/j.1438-8677.2010.00373.x. Plant Biol (Stuttg). 2010. PMID: 21040298 Review.
Cited by
-
Microbiota and Lipotoxicity.Adv Exp Med Biol. 2024;1460:357-372. doi: 10.1007/978-3-031-63657-8_12. Adv Exp Med Biol. 2024. PMID: 39287858 Review.
-
Cataloging the Genetic Response: Unveiling Drought-Responsive Gene Expression in Oil Tea Camellia (Camellia oleifera Abel.) through Transcriptomics.Life (Basel). 2024 Aug 8;14(8):989. doi: 10.3390/life14080989. Life (Basel). 2024. PMID: 39202731 Free PMC article.
-
A Comprehensive Review of Bioinformatics Tools for Genomic Biomarker Discovery Driving Precision Oncology.Genes (Basel). 2024 Aug 6;15(8):1036. doi: 10.3390/genes15081036. Genes (Basel). 2024. PMID: 39202397 Free PMC article. Review.
-
The application status of sequencing technology in global respiratory infectious disease diagnosis.Infection. 2024 Aug 16. doi: 10.1007/s15010-024-02360-4. Online ahead of print. Infection. 2024. PMID: 39152290 Review.
-
TabDEG: Classifying differentially expressed genes from RNA-seq data based on feature extraction and deep learning framework.PLoS One. 2024 Jul 22;19(7):e0305857. doi: 10.1371/journal.pone.0305857. eCollection 2024. PLoS One. 2024. PMID: 39037985 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources