Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Apr;65(4):2093-7.
doi: 10.1128/JVI.65.4.2093-2097.1991.

A negative regulatory element in the human papillomavirus type 16 genome acts at the level of late mRNA stability

I M Kennedy  1 , J K Haddow, J B Clements
Affiliations

A negative regulatory element in the human papillomavirus type 16 genome acts at the level of late mRNA stability

I M Kennedy et al. J Virol. 1991 Apr.

Abstract

A negative regulatory element present in the human papillomavirus type 16 genome has been characterized. Deletion analysis has localized the 5' end of the element to the late region of the genome at the extreme 3' end of the coding region of the L1 open reading frame, around the L1 stop codon, with the element extending into the L1 3' untranslated region. For the cell lines used, the element's function was independent of cell type, tissue, or species of origin, unlike papillomavirus infection, which is very dependent on such factors. By using an mRNA decay assay, we have determined that polyadenylated RNA containing the element is much less stable than polyadenylated RNA lacking the element. This indicates that the element acts as an mRNA instability element. The significance of A-rich, GU-rich, and AUG-rich sequences for the functioning of this human papillomavirus type 16 instability element is discussed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Cell Biol. 1988 Apr;8(4):1697-708 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Aug;82(15):4896-9 - PubMed
    1. Nucleic Acids Res. 1985 Feb 25;13(4):1347-68 - PubMed
    1. Nucleic Acids Res. 1985 Nov 11;13(21):7847-63 - PubMed
    1. J Virol. 1986 Jun;58(3):991-5 - PubMed

Publication types

LinkOut - more resources