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. 2008 Jun;5(6):527-9.
doi: 10.1038/nmeth.1211. Epub 2008 May 11.

Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples

Manuel F Juette  1 , Travis J Gould, Mark D Lessard, Michael J Mlodzianoski, Bhupendra S Nagpure, Brian T Bennett, Samuel T Hess, Joerg Bewersdorf
Affiliations

Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples

Manuel F Juette et al. Nat Methods. 2008 Jun.

Abstract

Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.

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Comment in

  • Super-resolution for a 3D world.
    Shaevitz JW. Shaevitz JW. Nat Methods. 2008 Jun;5(6):471-2. doi: 10.1038/nmeth0608-471. Nat Methods. 2008. PMID: 18511914 No abstract available.

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