doi: 10.1126/science.1846707.
Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq
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- PMID: 1846707
- DOI: 10.1126/science.1846707
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Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq
Science.
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Abstract
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by phospholipase C yields the second messengers inositol 1,4,5-trisphosphate (InsP3) and 1,2-diacylglycerol. This activity is regulated by a variety of hormones through G protein pathways. However, the specific G protein or proteins involved has not been identified. The alpha subunit of a newly discovered pertussis toxin-insensitive G protein (Gq) has recently been isolated and is now shown to stimulate the activity of polyphosphoinositide-specific phospholipase C (PI-PLC) from bovine brain. Both the maximal activity and the affinity of PI-PLC for calcium ion were affected. These results identify Gq as a G protein that regulates PI-PLC.
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