An in vivo and in vitro assessment of TOR signaling cascade in rainbow trout (Oncorhynchus mykiss)
- PMID: 18434442
- DOI: 10.1152/ajpregu.00146.2008
An in vivo and in vitro assessment of TOR signaling cascade in rainbow trout (Oncorhynchus mykiss)
Abstract
In mammals, feeding promotes protein accretion in skeletal muscle through a stimulation of the insulin- and amino acid- sensitive mammalian target of rapamycin (mTOR) signaling pathway, leading to the induction of mRNA translation. The purpose of the present study was to characterize both in vivo and in vitro the activation of several major kinases involved in the mTOR pathway in the muscle of the carnivorous rainbow trout. Our results showed that meal feeding enhanced the phosphorylation of the target of rapamycin (TOR), PKB, p70 S6 kinase, and eIF4E-binding protein-1, suggesting that the mechanisms involved in the regulation of mRNA translation are well conserved between lower and higher vertebrates. Our in vitro studies on primary culture of trout muscle cells indicate that insulin and amino acids regulate TOR signaling and thus may be involved in meal feeding effect in this species as in mammals. In conclusion, we report here for the first time in a fish species, the existence and the nutritional regulation of several major kinases involved in the TOR pathway, opening a new area of research on the molecular bases of amino acid utilization in teleosts.
Similar articles
-
Rainbow trout genetically selected for greater muscle fat content display increased activation of liver TOR signaling and lipogenic gene expression.Am J Physiol Regul Integr Comp Physiol. 2009 Nov;297(5):R1421-9. doi: 10.1152/ajpregu.00312.2009. Epub 2009 Aug 26. Am J Physiol Regul Integr Comp Physiol. 2009. PMID: 19710390
-
Hepatic protein kinase B (Akt)-target of rapamycin (TOR)-signalling pathways and intermediary metabolism in rainbow trout (Oncorhynchus mykiss) are not significantly affected by feeding plant-based diets.Br J Nutr. 2009 Dec;102(11):1564-73. doi: 10.1017/S000711450999095X. Epub 2009 Aug 10. Br J Nutr. 2009. PMID: 19664314 Clinical Trial.
-
Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation.Am J Physiol Endocrinol Metab. 2006 Apr;290(4):E631-42. doi: 10.1152/ajpendo.00460.2005. Epub 2005 Nov 1. Am J Physiol Endocrinol Metab. 2006. PMID: 16263769
-
Molecular mechanisms through which amino acids mediate signaling through the mammalian target of rapamycin.Curr Opin Clin Nutr Metab Care. 2004 Jan;7(1):39-44. doi: 10.1097/00075197-200401000-00008. Curr Opin Clin Nutr Metab Care. 2004. PMID: 15090902 Review.
-
Interplay between insulin and nutrients in the regulation of translation factors.Biochem Soc Trans. 2001 Aug;29(Pt 4):541-7. doi: 10.1042/bst0290541. Biochem Soc Trans. 2001. PMID: 11498025 Review.
Cited by
-
Involvement of Mechanistic Target of Rapamycin (mTOR) in Valine Orexigenic Effects in Rainbow Trout.Aquac Nutr. 2022 Sep 27;2022:7509382. doi: 10.1155/2022/7509382. eCollection 2022. Aquac Nutr. 2022. PMID: 36860456 Free PMC article.
-
Flesh quality loss in response to dietary isoleucine deficiency and excess in fish: a link to impaired Nrf2-dependent antioxidant defense in muscle.PLoS One. 2014 Dec 16;9(12):e115129. doi: 10.1371/journal.pone.0115129. eCollection 2014. PLoS One. 2014. PMID: 25514235 Free PMC article.
-
An insight on the impact of teleost whole genome duplication on the regulation of the molecular networks controlling skeletal muscle growth.PLoS One. 2021 Jul 22;16(7):e0255006. doi: 10.1371/journal.pone.0255006. eCollection 2021. PLoS One. 2021. PMID: 34293047 Free PMC article.
-
mRNA-seq reveals skeletal muscle atrophy in response to handling stress in a marine teleost, the red cusk-eel (Genypterus chilensis).BMC Genomics. 2015 Dec 1;16:1024. doi: 10.1186/s12864-015-2232-7. BMC Genomics. 2015. PMID: 26626593 Free PMC article.
-
Differences in growth, fillet quality, and fatty acid metabolism-related gene expression between juvenile male and female rainbow trout.Fish Physiol Biochem. 2015 Apr;41(2):533-47. doi: 10.1007/s10695-015-0027-z. Epub 2015 Feb 12. Fish Physiol Biochem. 2015. PMID: 25673423
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
